Del Río María Cristina, Martín Sergio, Quílez Joaquín, Molina José Manuel, Ferrer Otilia, Molina José Adrián, Melián Adrián, Ruiz Antonio
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Las Palmas de Gran Canaria (ULPGC), 35413 Arucas, Spain.
Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, 50013 Zaragoza, Spain.
Microorganisms. 2025 Mar 12;13(3):644. doi: 10.3390/microorganisms13030644.
The aim of this study was to analyse and characterise spp. in sheep and goats in Gran Canaria (Spain) and to identify the risks and economic factors related to the disease. During sampling, a semi-structured survey was conducted with farmers, and faecal samples were collected from lambs, goat kids, sheep, and adult goats from a total of 30 farms. Adult samples were examined microscopically for the presence of spp. oocysts, with only three positive samples being found in sheep and one in goats. The PCR of the gene was performed on all juvenile and adult samples, and positive samples from lambs (8.3%), sheep (6.9%), goat kids (23.3%), and goats (2.5%) were subjected to sequencing, detecting three of the most important species in small ruminants: , , and . By sequencing the PCR products, two subtypes of belonging to the IId family were identified, IIdA16G1 and IIdA23G1, with the latter being the most frequent. Although the prevalence of the disease was not very high, the zoonotic potential of and the limited awareness of the parasite among farmers make surveillance and health education focused on the control of this member of Apicomplexa necessary.
本研究的目的是分析和鉴定西班牙大加那利岛绵羊和山羊体内的球虫种类，并确定与该疾病相关的风险和经济因素。在采样过程中，对养殖户进行了半结构化调查，并从总共30个农场的羔羊、山羊羔、绵羊和成年山羊中采集了粪便样本。对成年样本进行显微镜检查，以检测球虫种类的卵囊，仅在绵羊中发现3份阳性样本，在山羊中发现1份阳性样本。对所有幼年和成年样本进行了该基因的PCR检测，并对来自羔羊（8.3%）、绵羊（6.9%）、山羊羔（23.3%）和山羊（2.5%）的阳性样本进行了测序，检测到小反刍动物中最重要的三种球虫种类：、和。通过对PCR产物进行测序，鉴定出属于IId家族的两种艾美耳球虫亚型，即IIdA16G1和IIdA23G1，后者最为常见。尽管该疾病的患病率不是很高，但艾美耳球虫的人畜共患病潜力以及养殖户对该寄生虫的认识有限，使得有必要开展以控制这种顶复门寄生虫为重点的监测和健康教育。
