Del Río María Cristina, Martín Sergio, Quílez Joaquín, Vergara-Castiblanco Claudia, Molina José Manuel, Ferrer Otilia, Conde Magnolia María, Molina José Adrián, Ruiz Antonio
Parasitology Unit, Department of Animal Pathology, Faculty of Veterinary Sciences, University of Las Palmas de Gran Canaria (ULPGC), Arucas, Las Palmas, Spain.
Medical and Surgery Unit, Department of Animal Pathology, Faculty of Veterinary Sciences, University of Las Palmas de Gran Canaria (ULPGC), Arucas, Las Palmas, Spain.
Int J Vet Sci Med. 2025 Feb 10;13(1):1-14. doi: 10.1080/23144599.2025.2460923. eCollection 2025.
spp. infections in calves cause serious economic losses in livestock and pose an important zoonotic risk. The aim of this study was to investigate the prevalence of spp. in cattle on the island of Gran Canaria. Faecal samples were collected from calves and adult cattle from a total of 15 farms, and a questionnaire survey was conducted to farmers. The presence of spp. oocysts in faeces was determined by microscopy, showing infection rates of 45.9% in calves and 4.1% in adults, with positive correlation with the faecal scores of infected animals ( < 0.0001). Samples were amplified by PCR targeting SSU rRNA, with positivity rates for calves and adults being 51.7% and 31.7%, respectively. The PCR-positive samples were further genotyped and sequenced for the 60 kDa glycoprotein gene (GP60) and the microsatellite TP14. Four species were identified (, and ), of which was the most frequent in calves (45.8%) and adults (29.2%). GP60 sequencing revealed that all samples belonged to the IId family, the most frequent subtypes being IIdA22G1 and IIdA23G1. Overall, the results of this study demonstrate a high occurrence of spp. in both calves and adult cattle, including the zoonotic IId family of . These findings have significant implications for cattle farming and public health. The lack of awareness among farmers regarding cryptosporidiosis highlights the need for caution to prevent epidemiological outbreaks that could impact both human and livestock health.
犊牛的隐孢子虫属感染会给畜牧业造成严重经济损失，并构成重要的人畜共患病风险。本研究的目的是调查大加那利岛牛群中隐孢子虫属的流行情况。从总共15个农场的犊牛和成年牛采集粪便样本，并对养殖户进行问卷调查。通过显微镜检查确定粪便中隐孢子虫属卵囊的存在情况，结果显示犊牛的感染率为45.9%，成年牛为4.1%，且与感染动物的粪便评分呈正相关（<0.0001）。通过针对小亚基核糖体RNA（SSU rRNA）的聚合酶链反应（PCR）对样本进行扩增，犊牛和成年牛的阳性率分别为51.7%和31.7%。对PCR阳性样本进一步进行60 kDa糖蛋白基因（GP60）和微卫星TP14的基因分型和测序。鉴定出四种隐孢子虫物种（、和），其中在犊牛（45.8%）和成年牛（29.2%）中最常见。GP60测序显示，所有隐孢子虫样本均属于IId家族，最常见的亚型为IIdA22G1和IIdA23G1。总体而言，本研究结果表明，犊牛和成年牛中隐孢子虫属的感染率都很高，包括人畜共患的隐孢子虫IId家族。这些发现对养牛业和公共卫生具有重要意义。养殖户对隐孢子虫病缺乏认识，这凸显了谨慎预防可能影响人类和牲畜健康的流行病学暴发的必要性。
