Qiu Chen, Pan Kailing, Wei Yuxuan, Zhou Xiaolin, Su Qingxian, Bi Xuejun, Ng Howyong
State and Local Joint Engineering Research Centre of Urban Wastewater Treatment and Reclamation in China, Qingdao University of Technology, Qingdao 266033, China.
Centre for Water Research, Advanced Institute of Natural Sciences, Beijing Normal University at Zhuhai, Zhuhai 519087, China.
Microorganisms. 2025 Mar 14;13(3):663. doi: 10.3390/microorganisms13030663.
This study investigated the mechanism by which N-acyl-homoserine lactone (AHL) signaling molecules influence ammonia-oxidizing microorganisms (AOMs) under inhibitory conditions. In laboratory-scale sequential batch reactors (SBRs), the effects of different AHLs (C6-HSL and C8-HSL) on the metabolic activity, microbial community structure, and quorum sensing (QS) system response of AOMs were examined. Caffeic acid, 1-octyne, and allylthiourea were used as ammoxidation inhibitors. The results indicated that under inhibitory conditions, AHLs effectively reduced the loss of ammonia oxidation activity and enhanced the resistance of AOMs to unfavorable environments. Additionally, AHLs enriched AOMs in the microbial community, wherein C6-HSL significantly increased the abundance of genes in AOMs. Furthermore, AHLs maintained the activity of QS-related genes and preserved the communication ability between microorganisms. Correlation analysis revealed a positive relationship between AOMs and QS functional bacteria, suggesting that AHLs can effectively regulate the ammonia oxidation process. Overall, exogenous AHLs can improve the metabolic activity and competitive survival of AOMs under inhibitory conditions.
本研究调查了N-酰基高丝氨酸内酯（AHL）信号分子在抑制条件下影响氨氧化微生物（AOMs）的机制。在实验室规模的序批式反应器（SBRs）中，研究了不同AHLs（C6-HSL和C8-HSL）对AOMs代谢活性、微生物群落结构和群体感应（QS）系统响应的影响。使用咖啡酸、1-辛炔和烯丙基硫脲作为氨氧化抑制剂。结果表明，在抑制条件下，AHLs有效减少了氨氧化活性的损失，增强了AOMs对不利环境的抗性。此外，AHLs在微生物群落中富集了AOMs，其中C6-HSL显著增加了AOMs中基因的丰度。此外，AHLs维持了QS相关基因的活性，并保留了微生物之间的通讯能力。相关性分析揭示了AOMs与QS功能细菌之间的正相关关系，表明AHLs可以有效调节氨氧化过程。总体而言，外源AHLs可以在抑制条件下提高AOMs的代谢活性和竞争生存能力。
