The Key Laboratory of Beijing for Water Quality Science & Water Environment Recovery Engineering, The College of Architecture and Civil Engineering, Beijing University of Technology, Beijing, China.
Beijing Zhibao Information Technology Co., Ltd, Beijing, China.
Water Environ Res. 2021 Sep;93(9):1576-1588. doi: 10.1002/wer.1539. Epub 2021 Mar 3.
This study investigated the relationship between acyl homoserine lactones (AHLs)-based quorum sensing (QS) and the properties of Anammox granular sludge at low temperatures (11-23°C). Results indicated that adding different concentrations of AHLs inhibitors reduced the content of N-hexanoyl-dl-homoserine lactone (C6-HSL) and N-octanoyl-dl-homoserinelactone (C8-HSL) in Anammox granules on different degrees at different operation temperatures, which led to the deterioration of granules stability and activity. The important role of endogenous C6-HSL and C8-HSL signals in maintaining Anammox granular sludge stability and activity in low-temperature conditions was revealed. In addition, in the process of reducing operation temperatures, another type of AHL signal (N-(3-oxooctanoyl)-l-homoserine lactone, 3OC8-HSL) was released by Anammox granules. The effects of exogenous C8-HSL on the strength, average diameter, and density of Anammox granules were closely related to the operation temperature. When the operation temperature ranged from 11°C to 16°C, the stability of granules could be significantly improved by exogenous C8-HSL. In addition, the addition of C6-HSL and 3OC8-HSL promoted the activity of Anammox granules when the operation temperatures of the reactors were 11-23°C. This study proposed a novel approach to improve the properties of Anammox granules at low temperatures from the perspective of QS. PRACTITIONER POINTS: Endogenous AHLs played an important role in maintaining the activity and stability of Anammox granules at 11-23°C. Exogenous C8-HSL improved the granules stability at the low temperature of 11-16°C. Exogenous C6-HSL or 3OC8-HSL promoted the granules activity at 11-23°C. Supply a novel way to improve the Anammox granules performance at low temperatures.
本研究考察了酰基高丝氨酸内酯（AHLs）为基础的群体感应（QS）与 Anammox 颗粒污泥在低温（11-23°C）下性能之间的关系。结果表明，在不同的操作温度下，添加不同浓度的 AHLs 抑制剂会不同程度地降低 Anammox 颗粒中 N-己酰基-DL-高丝氨酸内酯（C6-HSL）和 N-辛酰基-DL-高丝氨酸内酯（C8-HSL）的含量，从而导致颗粒稳定性和活性恶化。揭示了内源性 C6-HSL 和 C8-HSL 信号在低温条件下维持 Anammox 颗粒污泥稳定性和活性的重要作用。此外，在降低操作温度的过程中，Anammox 颗粒释放了另一种 AHL 信号（N-（3-氧代辛酰基）-L-高丝氨酸内酯，3OC8-HSL）。外源性 C8-HSL 对 Anammox 颗粒强度、平均直径和密度的影响与操作温度密切相关。当操作温度范围为 11°C 至 16°C 时，外源性 C8-HSL 可显著提高颗粒的稳定性。此外，当反应器的操作温度为 11-23°C 时，添加 C6-HSL 和 3OC8-HSL 可促进 Anammox 颗粒的活性。本研究从 QS 的角度提出了一种提高低温下 Anammox 颗粒性能的新方法。
内源性 AHLs 在 11-23°C 时对维持 Anammox 颗粒的活性和稳定性起着重要作用。
外源性 C8-HSL 可提高 11-16°C 低温下颗粒的稳定性。
外源性 C6-HSL 或 3OC8-HSL 可促进 11-23°C 下颗粒的活性。
提供了一种提高低温下 Anammox 颗粒性能的新方法。
