de Carvalho Anne Kerollen Pinheiro, Cruz Ana Cecília Ribeiro, Quaresma Juarez Antônio Simões, Filho Arnaldo Jorge Martins, Durans Darlene de Brito Simith, Amador Neto Orlando Pereira, de Lima Ligia do Socorro Oliveira, Assunçao Norma Suely de Carvalho Fonseca, Franco Edna Cristina Santos, Cohen Patrícia Brazão, da Silva Eliana Vieira Pinto
Instituto Evandro Chagas, Seção de Arbovirologia e Febres Hemorrágicas, Ananindeua 67030-000, PA, Brazil.
Department of Pathology, State University of Pará, Belém 66075-110, PA, Brazil.
Microorganisms. 2025 Mar 18;13(3):678. doi: 10.3390/microorganisms13030678.
To investigate the association between miscarriage and ZIKV and CHIKV infection. The study population consisted of pregnant women who had miscarriages between 2015, 2016 and 2017, comprising a total of 30 women who were treated at the Santa Casa de Misericórdia do Pará Foundation (FSCMPA). The processed samples came from already paraffinized material containing placental and fetal remains, where they were tested with hematoxylin-eosin and immunohistochemistry for ZIKV and CHIKV. Regarding the sociodemographic, clinical and obstetric characteristics of the patients, they correspond to the age group between 20 and 29 years of age; of brown color; women who had abortions for the first time; miscarriages occurring in the first trimester of pregnancy; women belonging to the metropolitan region of Belém; diagnosed with incomplete abortion and who had undergone uterine curettage procedure. Regarding the histopathologic and immunohistochemical findings, an inflammatory infiltrate rich in neutrophils and lymphocytes, among others, was found in the endometrial fragments and chorionic membranes. In addition, placental areas consisting of edema, necrosis and hemorrhage were found. The study identified ZIKV and CHIKV in 40% (n = 12) of samples from spontaneous abortion specimens, and CHIKV was the most prevalent virus in the study, representing 36.11% of the total specimens, with reddish granular material in the cytoplasm of decidua cells and placental villi suggesting that the viruses may be present in these regions of the placenta.
为研究流产与寨卡病毒（ZIKV）和基孔肯雅病毒（CHIKV）感染之间的关联。研究人群包括2015年、2016年和2017年间流产的孕妇，共有30名在帕拉州慈悲圣母基金会（FSCMPA）接受治疗的女性。处理后的样本来自已石蜡包埋的含有胎盘和胎儿残骸的材料，对其进行苏木精-伊红染色及针对ZIKV和CHIKV的免疫组织化学检测。关于患者的社会人口学、临床和产科特征，她们年龄在20至29岁之间；肤色为棕色；首次流产；流产发生在妊娠早期；属于贝伦大都市区的女性；诊断为不全流产且接受过刮宫手术。关于组织病理学和免疫组织化学结果，在子宫内膜碎片和绒毛膜中发现了富含中性粒细胞和淋巴细胞等的炎性浸润。此外，还发现了由水肿、坏死和出血组成的胎盘区域。该研究在40%（n = 12）的自然流产标本样本中检测到ZIKV和CHIKV，且CHIKV是该研究中最常见的病毒，占总标本的36.11%，蜕膜细胞和胎盘绒毛细胞质中的红色颗粒物质表明病毒可能存在于胎盘的这些区域。
