Chen Lifei, Li Lusheng, Wang Guiying, Xu Meng, Xin Yizhen, Song Hanhan, Liu Jiale, Fu Jiani, Yang Qi, Tian Qile, Wang Yuxi, Sun Haoyang, Lin Jianqun, Chen Linxu, Zhang Jiang, Lin Jianqiang
College of Agriculture and Biology, Shandong Province Engineering Research Center of Black Soldier Fly Breeding and Organic Waste Conversion, Liaocheng University, Liaocheng 252000, China.
State Key Laboratory of Microbial Technology, Microbial Technology Institute, Shandong University, Qingdao 266237, China.
Microorganisms. 2025 Mar 19;13(3):685. doi: 10.3390/microorganisms13030685.
A microbial nest system (MNS) represents a novel and efficient approach to treating solid-liquid mixtures from pig farming instead of the conventional method, which separates the solid and liquid at first using centrifugation before treating the solid and liquid. However, the key environmental factors influencing the efficiency of this system and the microbial structure are still not clear. This study aimed to elucidate the changes in an MNS considering physicochemical properties, spectral analysis, and correlations between microbial community structures and environmental factors during the treatment. The results showed that the MNS underwent three temperature stages during the treatment process of piggery slurry: a warming period, a high-temperature period, and a cooling period. In the high-temperature period, the most abundant bacterium was , with a relative abundance of 22.16%, and dominated the fungal community with a relative abundance of 11.40%. Moreover, the moisture content, pH value, and electrical conductivity (EC) exhibited an increasing trend, whereas the carbon-to-nitrogen (C/N) ratio and the ratio of ammonia nitrogen to nitrate nitrogen (NH-N/NO-N) showed a decreasing trend. The accumulation of humic acid and fulvic acid suggested that the humification process of organic matter was occurring. The moisture content and C/N ratio were identified as crucial factors influencing the bacterial and fungal community structures, respectively. This study provides a theoretical basis for enhancing the efficiency of piggery slurry treatment using an MNS and rational optimisation of the associated processes.
微生物巢系统（MNS）是一种用于处理养猪场固液混合物的新颖且高效的方法，而不是传统方法，传统方法是先通过离心分离固体和液体，然后再分别处理固体和液体。然而，影响该系统效率和微生物结构的关键环境因素仍不明确。本研究旨在阐明在处理过程中，考虑物理化学性质、光谱分析以及微生物群落结构与环境因素之间的相关性时，MNS的变化情况。结果表明，在猪粪污水处理过程中，MNS经历了三个温度阶段：升温期、高温期和冷却期。在高温期，最丰富的细菌是 ，相对丰度为22.16%， 以11.40%的相对丰度主导真菌群落。此外，水分含量、pH值和电导率（EC）呈上升趋势，而碳氮比（C/N）和氨氮与硝酸盐氮的比值（NH-N/NO-N）呈下降趋势。腐殖酸和富里酸的积累表明有机物的腐殖化过程正在发生。水分含量和C/N比分别被确定为影响细菌和真菌群落结构的关键因素。本研究为提高利用MNS处理猪粪污水的效率及合理优化相关工艺提供了理论依据。
