Insights into a Novel and Efficient Microbial Nest System for Treating Pig Farm Wastewater.

A microbial nest system (MNS) represents a novel and efficient approach to treating solid-liquid mixtures from pig farming instead of the conventional method, which separates the solid and liquid at first using centrifugation before treating the solid and liquid. However, the key environmental factors influencing the efficiency of this system and the microbial structure are still not clear. This study aimed to elucidate the changes in an MNS considering physicochemical properties, spectral analysis, and correlations between microbial community structures and environmental factors during the treatment. The results showed that the MNS underwent three temperature stages during the treatment process of piggery slurry: a warming period, a high-temperature period, and a cooling period. In the high-temperature period, the most abundant bacterium was , with a relative abundance of 22.16%, and dominated the fungal community with a relative abundance of 11.40%. Moreover, the moisture content, pH value, and electrical conductivity (EC) exhibited an increasing trend, whereas the carbon-to-nitrogen (C/N) ratio and the ratio of ammonia nitrogen to nitrate nitrogen (NH-N/NO-N) showed a decreasing trend. The accumulation of humic acid and fulvic acid suggested that the humification process of organic matter was occurring. The moisture content and C/N ratio were identified as crucial factors influencing the bacterial and fungal community structures, respectively. This study provides a theoretical basis for enhancing the efficiency of piggery slurry treatment using an MNS and rational optimisation of the associated processes.