Wen Ping, Wang Yueqiang, Huang Wenfeng, Wang Weiwu, Chen Tao, Yu Zhen
National-Regional Joint Engineering Research Center for Soil Pollution Control and Remediation in South China, Guangdong Key Laboratory of Integrated Agro-Environmental Pollution Control and Management, Institute of Eco-Environmental and Soil Sciences, Guangdong Academy of Sciences, Guangzhou, China.
Environmental Research Institute, School of Environment, Guangdong Provincial Key Laboratory of Chemical Pollution and Environmental Safety and MOE Key Laboratory of Theoretical Chemistry of Environment, South China Normal University, Guangzhou, China.
Front Microbiol. 2022 May 4;13:886161. doi: 10.3389/fmicb.2022.886161. eCollection 2022.
Ectopic fermentation system (EFS) is an effective technology for treating mass livestock manure. However, the associations between microbial communities and substance transformation remain controversial. This study aimed to investigate chicken manure EFS lasting 170 days using 16S rRNA sequencing and electrochemical, spectroscopic, and chromatographic analyses. The results showed a noticeable transformation of protein-like substances into humus-like substances. Meanwhile, the electron-accepting capacity increased persistently, effectively reflecting the humification of organic substances. The contents of phenols that promoted electron transfer continued to increase from 2.80 to 6.00%, which could be used as a maturity indicator for EFS. During the heating period, the dominant microbial communities were and , whereas thermotolerant bacteria and were significantly enriched from 1.64 to 50.15% during the continuous thermophilic period of EFS. The correlation analysis manifested that these thermotolerant bacteria were the major functional bacteria for the formation of phenols and the key to driving the humification of organic substances. This study provides insights into understanding the humification mechanisms and implementing regulatory strategies in EFS.
异位发酵系统（EFS）是一种处理大量畜禽粪便的有效技术。然而，微生物群落与物质转化之间的关联仍存在争议。本研究旨在通过16S rRNA测序以及电化学、光谱和色谱分析，对持续170天的鸡粪EFS进行研究。结果表明，类蛋白物质显著转化为类腐殖质物质。同时，电子接受能力持续增加，有效反映了有机物的腐殖化过程。促进电子转移的酚类物质含量从2.80%持续增加到6.00%，可作为EFS的成熟度指标。在加热期，优势微生物群落为 和 ，而在EFS的持续嗜热期，耐热细菌 和 从1.64%显著富集到50.15%。相关性分析表明，这些耐热细菌是酚类物质形成的主要功能细菌，也是推动有机物腐殖化的关键。本研究为理解EFS中的腐殖化机制和实施调控策略提供了见解。
