Sinisi Antonio Agostino, Rossi Valentina, Martino Marco De, Esposito Francesco, Chieffi Paolo
Department of Advanced Medical and Surgical Sciences, University of Campania "Luigi Vanvitelli", Naples, Italy.
Institute of Endocrinology and Experimental Oncology (IEOS) "G. Salvatore", National Research Council (CNR), Naples, Italy.
GHM Open. 2024 Jul 31;4(1):37-41. doi: 10.35772/ghmo.2023.01021.
In steroidogenic tissues, a novel class of angiogenic molecules known as endocrine gland-derived vascular endothelial growth factors (EG-VEGF)/prokineticins are primarily produced. Here, we investigated how EG-VEGF/ PROK1, a member of PROKs family, and its receptor are able to affect cellular motility in both non-neoplastic and cancerous human prostate cells. Using Western blot and motility test studies, EPN cells, a non-transformed cell line and Cancer Epithelial Prostatic Cells (CEPC) were employed as cellular models in the current investigation. Western blot examination of EPN normal prostate cells treated with EG-VEGF/PROK1 revealed that ERK1/2 was rapidly phosphorylated within 5, 10, and 20 minutes, while CEPC had high and sustained ERK1/2 activity at the same periods. Then, compared to normal EPN prostate cells, CEPC treated with EG-VEGF/PROK1 for up to 72 hours demonstrated enhanced cell motility. Based on our findings, EG-VEGF/PROK1 may play a role in prostate cancer progression by controlling angiogenesis and the motility of metastatic cells in CEPC cells, likely as a consequence of ERK1/2 activation, as contrasted to EPN normal prostate cells.
在类固醇生成组织中，主要产生一类被称为内分泌腺源性血管内皮生长因子（EG-VEGF）/促动力蛋白的新型血管生成分子。在此，我们研究了促动力蛋白家族成员EG-VEGF/PROK1及其受体如何影响非肿瘤性和癌性人前列腺细胞的细胞运动性。利用蛋白质免疫印迹法和运动性测试研究，在本研究中采用非转化细胞系EPN细胞和前列腺癌上皮细胞（CEPC）作为细胞模型。用EG-VEGF/PROK1处理EPN正常前列腺细胞的蛋白质免疫印迹检测显示，ERK1/2在5、10和20分钟内迅速磷酸化，而CEPC在同一时期具有高水平且持续的ERK1/2活性。然后，与正常EPN前列腺细胞相比，用EG-VEGF/PROK1处理长达72小时的CEPC表现出增强的细胞运动性。基于我们的发现，EG-VEGF/PROK1可能通过控制CEPC细胞中的血管生成和转移细胞的运动性在前列腺癌进展中发挥作用，这可能是ERK1/2激活的结果，与EPN正常前列腺细胞形成对比。
