Staibano Stefania, Mascolo Massimo, Mancini Francesco Paolo, Kisslinger Annamaria, Salvatore Gaetano, Di Benedetto Maria, Chieffi Paolo, Altieri Vincenzo, Prezioso Domenico, Ilardi Gennaro, De Rosa Gaetano, Tramontano Donatella
Department of Biomorphological and Functional Sciences, Pathology Section, University of Naples Federico II, Naples, Italy.
Histopathology. 2009 Apr;54(5):580-9. doi: 10.1111/j.1365-2559.2009.03266.x. Epub 2009 Mar 20.
Prostatic cancer may remain organ-confined indefinitely; in a number of patients, however it gives rise to clinical symptoms and death. The biological behaviour of this tumour mostly remains difficult to predict. A promising tool for diagnosis and prognosis of some human tumours is the chromatin assembly factor-1 (CAF-1), involved in the control of higher order chromatin organization. The aim was to explore the role of CAF-1/p60 protein as a new prognostic marker for prostatic cancer.
The expression of CAF-1/p60 was evaluated by immunohistochemistry and Western blotting in a selected series of prostatic cancers and in prostatic cancer cell lines. Results were compared with clinicopathological data and outcome of patients. CAF-1/p60 was expressed in all cases, with a linear increase from low-grade tumours (Gleason score <7) to high-grade prostatic cancers (Gleason score >7). By comparing results with follow-up data, a significant association between overexpression of CAF-1/p60 and unfavourable behaviour of prostatic cancer emerged, and its predictive value was independent of classical prognostic factors.
In our series of cases, overexpression of CAF-1/p60 characterized prostatic cancers with a worse prognosis. CAF-1/p60 has a potential role as a new reliable prognostic biomarker for prostatic cancer.
前列腺癌可能长期局限于器官内；然而，在一些患者中，它会引发临床症状并导致死亡。这种肿瘤的生物学行为大多仍难以预测。染色质组装因子-1（CAF-1）参与高阶染色质组织的控制，是诊断和预测某些人类肿瘤的一种有前景的工具。本研究旨在探讨CAF-1/p60蛋白作为前列腺癌新的预后标志物的作用。
通过免疫组织化学和蛋白质印迹法评估了一系列选定的前列腺癌组织及前列腺癌细胞系中CAF-1/p60的表达情况。将结果与患者的临床病理数据及预后进行比较。所有病例均表达CAF-1/p60，其表达水平从低级别肿瘤（Gleason评分<7）到高级别前列腺癌（Gleason评分>7）呈线性增加。通过将结果与随访数据进行比较，发现CAF-1/p60的过表达与前列腺癌的不良行为之间存在显著关联，且其预测价值独立于经典预后因素。
在我们的病例系列中，CAF-1/p60的过表达是预后较差的前列腺癌的特征。CAF-1/p60作为前列腺癌一种新的可靠预后生物标志物具有潜在作用。
