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A spectrophotometric resin competition procedure to determine dissociation constants for metal ion-nucleotide complexes.

作者信息

Jenkins W T

出版信息

Anal Biochem. 1985 Mar;145(2):362-6. doi: 10.1016/0003-2697(85)90375-6.

DOI:10.1016/0003-2697(85)90375-6
PMID:4014667
Abstract

The resin competition method for determining the dissociation constants of metal ion-nucleotide complexes was modified to take into account the fact that some metal nucleotide complexes are anionic and thus, like the free nucleotide, will bind to the resin. A simple, rapid spectrophotometric titration procedure is given for the determination of both the metal ion-nucleotide complex dissociation constant and the ratio of the affinities of the nucleotide and its complex for the resin.

摘要

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