Clary L G, Voige W H, Leary J J
Anal Biochem. 1983 Feb 15;129(1):228-34. doi: 10.1016/0003-2697(83)90074-x.
Two data reduction methods that can be used to calculate formation constants for nucleotide-cation complexes are described. Both methods are used to analyze data obtained by an anion-exchange resin method, and either method can improve the accuracy of the calculated formation constants by more than 50%. The key to this significant improvement in accuracy is the realization that the equation for the mathematical model describing such systems is always nonlinear in terms of the formation constants and, in the general case, is higher order in the cation concentration. There are two major reasons for the improved quality of the results associated with the new model. First, successive linear extrapolations are eliminated, and error propagation is reduced. Second, all of the data are used for the simultaneous calculation of all formation constants, and the uncertainty due to random experimental errors is minimized.
描述了两种可用于计算核苷酸 - 阳离子配合物形成常数的数据约简方法。这两种方法都用于分析通过阴离子交换树脂法获得的数据，并且任何一种方法都可以将计算得到的形成常数的准确性提高超过50%。准确性显著提高的关键在于认识到描述此类系统的数学模型方程在形成常数方面始终是非线性的，并且在一般情况下，在阳离子浓度方面是高阶的。与新模型相关的结果质量提高有两个主要原因。首先，消除了连续线性外推，并减少了误差传播。其次，所有数据都用于同时计算所有形成常数，并且由于随机实验误差导致的不确定性被最小化。
