[4-¹⁴C]草酰乙酸的合成、储存及稳定性
Synthesis, storage, and stability of [4-14C]oxaloacetic acid.
作者信息
Hatch M D, Heldt H W
出版信息
Anal Biochem. 1985 Mar;145(2):393-7. doi: 10.1016/0003-2697(85)90379-3.
A simple procedure for preparing [4-14C]oxaloacetic acid based on the reaction between [14C]HCO-3 and phosphoenolpyruvate catalyzed by phosphoenolpyruvate carboxylase is described. A simple method for preparing highly purified phosphoenolpyruvate carboxylase from maize leaves is described and the degradation of oxaloacetate under conditions of varying pH and divalent metal ion concentration is reported on. [4-14C]Oxaloacetic acid is stable for several months in 0.1 M HCl solution at -80 degrees C.
描述了一种基于磷酸烯醇丙酮酸羧化酶催化的[¹⁴C]HCO₃⁻与磷酸烯醇丙酮酸之间的反应制备[4-¹⁴C]草酰乙酸的简单方法。描述了一种从玉米叶片中制备高度纯化的磷酸烯醇丙酮酸羧化酶的简单方法，并报道了在不同pH和二价金属离子浓度条件下草酰乙酸的降解情况。[4-¹⁴C]草酰乙酸在-80℃的0.1M HCl溶液中可稳定保存数月。
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