利用离心进行塑性压缩构建仿生肿瘤微球模型的方案。
Protocol for biomimetic tumoroid models by plastic compression using centrifugation.
作者信息
Devereaux Sam, Lam Ashley, Upadhyay Anuja, Fallows Megan, Qiu Lianqi, Pan Xueni, Cheema Umber
机构信息
UCL Centre for 3D Models of Health and Disease, UCL Division of Surgery and Interventional Science, Faculty of Medical Sciences, Charles Bell House, University College London, 43-45 Foley Street, London W1W 7TY, UK.
Department of Computing, Goldsmiths, University of London, 25 St James's, London SE14 6AD, UK.
出版信息
STAR Protoc. 2025 Jun 20;6(2):103718. doi: 10.1016/j.xpro.2025.103718. Epub 2025 Mar 26.
Here, we present a protocol for engineering biomimetic tumoroid models by plastic compression using centrifugation. We describe steps for generating multi-compartment tumor-stroma models by mixing cells into a collagen hydrogel crosslinked at 37°C and centrifuging the hydrogel. We then detail procedures for generating compartmentalized models and encapsulating the final layered hydrogel containing a 96-well tumor mass in a 24-well stroma. This protocol increases collagen density and improves mechanical properties of collagen hydrogels.
在此，我们展示了一种通过离心进行塑性压缩来构建仿生肿瘤类器官模型的方案。我们描述了将细胞混入在37°C交联的胶原水凝胶中并对水凝胶进行离心以生成多隔室肿瘤-基质模型的步骤。然后，我们详细介绍了生成隔室化模型以及将包含96孔肿瘤块的最终分层水凝胶封装在24孔基质中的程序。该方案提高了胶原密度并改善了胶原水凝胶的机械性能。
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