Chen H J, Bradley C J, Meites J
Cancer Res. 1977 Jan;37(1):64-6.
Twice-daily injections of three different doses of synthetic thyrotropin-releasing hormone (TRH), a hormone normally produced by the hypothalamus, produced significant increases in size and number of 7,12-dimethylbenz(a)anthracene-induced mammary cancers over 0.87% NaCl solution-injected control rats. When thyroidectomized rats, bearing 7,12-dimethylbenz(a)anthracene-induced mammary tumors were given the same twice-daily injections of TRH, mammary tumor growth was increased to the same extent as in intact rats given TRH, showing that the effects of TRH were not exerted via stimulation of thyroid function. The TRH-induced increments in mammary tumor growth were accompanied by significant increases in serum prolactin levels over 0.87% NaCl solution-injected controls. A single daily injection of 2-bromo-alpha-ergocryptine (CB-154), a prolactin-release inhibitor completely blocked TRH-induced mammary tumor growth and reduced serum prolactin values. These results indicate that a twice-daily pulse of TRH can stimulate mammary tumor growth by releasing prolactin from the anterior pituitary.
每日两次注射三种不同剂量的合成促甲状腺激素释放激素（TRH），该激素通常由下丘脑产生，与注射0.87%氯化钠溶液的对照大鼠相比，可使7,12-二甲基苯并(a)蒽诱导的乳腺癌的大小和数量显著增加。当给患有7,12-二甲基苯并(a)蒽诱导的乳腺肿瘤的甲状腺切除大鼠每日两次注射相同剂量的TRH时，乳腺肿瘤生长增加到与给予TRH的完整大鼠相同的程度，表明TRH的作用不是通过刺激甲状腺功能发挥的。与注射0.87%氯化钠溶液的对照相比，TRH诱导的乳腺肿瘤生长增加伴随着血清催乳素水平的显著升高。每日单次注射2-溴-α-麦角隐亭（CB-154），一种催乳素释放抑制剂，可完全阻断TRH诱导的乳腺肿瘤生长并降低血清催乳素值。这些结果表明，每日两次的TRH脉冲可通过从垂体前叶释放催乳素来刺激乳腺肿瘤生长。
