Ren Chunxiao, Zhang Meng, Zheng Na, Liu Benhua, Tang Ju, Tang Jianing, Zhang Fan, Chen Guo
Kunming Key Laboratory of Energy Materials Chemistry, Yunnan Minzu University, PR China.
Kunming Key Laboratory of Energy Materials Chemistry, Yunnan Minzu University, PR China; Yumnan Key Laboratory of Unmanned Autonomous System, School of Electrical and Information Technology, Yumnan Minzu University, Kunming 650500 Yumna, PR China; Yunnan Provincial Department of Education Engineering Research Center for Building Digital and Energy Saving Technology, Yunnan Minzu University, Kunming 650500 Yunnan, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2025 Sep 5;337:126084. doi: 10.1016/j.saa.2025.126084. Epub 2025 Mar 20.
Rutin has antioxidant, antitumor, antibacterial, antiviral, anti-aging, and analgesic effects, and it is a drug used to treat various circulatory disorders. However, rutin overdosage can seriously harm the health. The rutin detection method has drawbacks, including expensive costs, low sensitivity, and complicated steps. Therefore, given that carbon dots have superior fluorescence characteristics, the development of nanofluorescent probes for rutin detection is the research motivation of this article. N-CDs and N, B-CDs were produced by a hydrothermal approach using o-phenylenediamine, glucose, urea, and boron oxide, commonly available in the laboratory. The morphology and composition of CDs have been analyzed. According to the findings, the surfaces of the N-CDs and N, B-CDs were abundant in functional groups that contained oxygen, and they were spherical, uniform in size, and well distributed, which is beneficial for enhancing its fluorescence response to the detection object. In addition, two kinds of CDs emit bright yellow and blue-green fluorescence under the ultraviolet lamp at 365 nm. Rutin was detected in solution using synthesized N-CDs and N, B-CDs as fluorescent probes. Rutin was discovered to have a fluorescence quenching effect on N-CDs and N, B-CDs. Within a specific concentration range, the N-CDs and N, B-CDs fluorescence intensities demonstrated an excellent linear relationship with the rutin concentration, and the corresponding correlation coefficients R were 0.9897 and 0.9409, respectively. To verify the detection accuracy of the experiment, we used the random forest algorithm to predict the detection model. The results showed that the experimental and predicted values were coincident, suggesting the possibility of using N-CDs and N, B-CDs as fluorescent probes to detect rutin. This work offers experimental procedures and data references to create doping CDs with superior fluorescence qualities. It broadens the use of doped CDs in the detection of rutin.
芦丁具有抗氧化、抗肿瘤、抗菌、抗病毒、抗衰老和镇痛作用，是一种用于治疗各种循环系统疾病的药物。然而，芦丁过量会严重损害健康。芦丁检测方法存在缺点，包括成本高、灵敏度低和步骤复杂。因此，鉴于碳点具有优异的荧光特性，开发用于芦丁检测的纳米荧光探针是本文的研究动机。采用水热法，以实验室常用的邻苯二胺、葡萄糖、尿素和氧化硼制备了氮掺杂碳点（N-CDs）和氮、硼共掺杂碳点（N,B-CDs）。对碳点的形貌和组成进行了分析。结果表明，N-CDs和N,B-CDs表面富含含氧官能团，呈球形，尺寸均匀，分布良好，有利于增强其对检测对象的荧光响应。此外，两种碳点在365 nm紫外灯下发出亮黄色和蓝绿色荧光。以合成的N-CDs和N,B-CDs为荧光探针检测溶液中的芦丁。发现芦丁对N-CDs和N,B-CDs有荧光猝灭作用。在特定浓度范围内，N-CDs和N,B-CDs的荧光强度与芦丁浓度呈现出良好的线性关系，相应的相关系数R分别为0.9897和0.9409。为验证实验的检测准确性，我们使用随机森林算法预测检测模型。结果表明，实验值与预测值吻合，表明使用N-CDs和N,B-CDs作为荧光探针检测芦丁的可能性。这项工作为制备具有优异荧光性能的掺杂碳点提供了实验步骤和数据参考。它拓宽了掺杂碳点在芦丁检测中的应用。
