Lu Ziyue, He Sun, Adnan Ali, Fan Wenyu, Sheng Jinliang, Sun Yanming, Zhang Yanbing, Wang Gang
College of Animal Science and Technology, Shihezi University, Shihezi, Xinjiang, China.
TECON Pharmaceutical Co.,Ltd, Urumqi, 830011, China.
Sci Rep. 2025 Mar 27;15(1):10583. doi: 10.1038/s41598-025-95405-z.
A batch of sheep in a sheep farm in Xinjiang, China, died suddenly; a bacterial strain was isolated from the abdominal fluid of the sick and dead sheep, and identified as Rothia nasimurium by 16S sequencing, and the strain Y1 was subjected to drug sensitivity test with Draft gene sequencing. The results of the drug sensitivity test revealed the strain's resistance to 9 antibiotics, with sensitivity exhibited solely towards amikacin and vancomycin. Phylogenetic tree analysis confirmed that it was related to Rothia nasimurium strain E1706032 and Rothia sp.SD9660Na. The draft genome sequencing results showed that the total length of the gene was 2,387,685 bp, and the GC content was 59.35%. VFDB database analysis identified 112 annotated genes in Y1, including those related to bacterial adhesion, regulation, nutrient metabolism factors, hemolysin, immunomodulation, and iron uptake proteins. CARD database analysis showed that Y1 was resistant to a variety of antibiotics such as glycopeptides, tetracyclines, aminoglycosides and polypeptides. Animal pathogenicity tests have shown that Y1 can cause lung damage, coat loss and skin inflammation. This study revealed a series of virulence and drug resistance genes and pathogenicity of Y1. The results of this study have important reference value for prevention and treatment of Rothia infection in the future.
中国新疆某养羊场一批羊突然死亡；从病死羊腹腔液中分离出一株细菌，经16S测序鉴定为鼻腔罗思氏菌，并对该Y1菌株进行了耐药基因测序药敏试验。药敏试验结果显示该菌株对9种抗生素耐药，仅对阿米卡星和万古霉素敏感。系统发育树分析证实它与鼻腔罗思氏菌菌株E1706032和罗思氏菌属SD9660Na相关。基因组草图测序结果显示，基因全长2387685bp，GC含量为59.35%。VFDB数据库分析在Y1中鉴定出112个注释基因，包括与细菌黏附、调控、营养代谢因子、溶血素、免疫调节和铁摄取蛋白相关的基因。CARD数据库分析表明，Y1对多种抗生素耐药，如糖肽类、四环素类、氨基糖苷类和多肽类。动物致病性试验表明，Y1可导致肺部损伤、被毛脱落和皮肤炎症。本研究揭示了Y1的一系列毒力、耐药基因及致病性。本研究结果对今后鼻腔罗思氏菌感染的防治具有重要参考价值。
