Babaei Lavin, Sharifani M Mehdi, Darvishzadeh Reza, Abbaspour Naser, Henareh Mashhid
Department of Horticulture, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Department of Plant Production and Genetics, Faculty of Agriculture, Urmia University, Urmia, Iran.
BMC Plant Biol. 2025 Mar 28;25(1):397. doi: 10.1186/s12870-025-06408-x.
Drought is one of the crucial abiotic stresses which affects growth, development, and performance of pear trees. This research was performed to investigate responses of five pear species including Pyrus communis L., Pyrus boissieriana Bushe., Pyrus glabra Boiss., Pyrus syriaca Boiss., and Pyrus salicifolia Pall. to different levels of drought stress. The potted trees were irrigated with water volume of 100%, 60%, or 30% of field capacity (FC) during 90 days. Based on the visual observation, the plant growth was restricted by severe drought in all species. Malondialdehyde (MDA) and glycine betaine (GB) contents, as well as the ascorbate peroxidase (APX), guaiacol peroxidase (GPX) and catalase (CAT) activities were indicated an uprising behavior under drought stress. Principal component analysis (PCA) analysis revealed P. glabra as tolerant and P. communis as sensitive to drought stress. The expression rates of stress-responsive transcription factors (TFs); WRKY29 and DREB6 and their responding genes, including LEA29 and Dehydrin1, were analyzed in the two differentially drought-responding pear species i.e., P. glabra and P. communis. The expression of the studied TFs was induced when both species were exposed to higher drought levels. The species P. glabra exhibited better osmoregulation, antioxidant response, and higher up-regulation of WRKY29, DREB6, LEA29 and Dehydrin1. In conclusion, among the studied pear species, P. glabra could best tolerate drought stress by boosting protective mechanisms.
干旱是影响梨树生长、发育和性能的关键非生物胁迫之一。本研究旨在调查五个梨品种，即西洋梨（Pyrus communis L.）、布氏梨（Pyrus boissieriana Bushe.）、光叶梨（Pyrus glabra Boiss.）、叙利亚梨（Pyrus syriaca Boiss.）和柳叶梨（Pyrus salicifolia Pall.）对不同程度干旱胁迫的响应。在90天内，对盆栽树分别浇灌田间持水量（FC）100%、60%或30%的水量。基于视觉观察，所有品种的植物生长均受到严重干旱的限制。丙二醛（MDA）和甘氨酸甜菜碱（GB）含量以及抗坏血酸过氧化物酶（APX）、愈创木酚过氧化物酶（GPX）和过氧化氢酶（CAT）活性在干旱胁迫下均呈上升趋势。主成分分析（PCA）显示光叶梨对干旱胁迫具有耐受性，而西洋梨对干旱胁迫敏感。在两个对干旱响应不同的梨品种，即光叶梨和西洋梨中，分析了胁迫响应转录因子（TFs）WRKY29和DREB6及其响应基因，包括LEA29和脱水素1的表达率。当两个品种都暴露于更高的干旱水平时，所研究的TFs的表达被诱导。光叶梨品种表现出更好的渗透调节、抗氧化反应以及WRKY29、DREB6、LEA29和脱水素1的更高上调。总之，在所研究的梨品种中，光叶梨通过增强保护机制能够最佳地耐受干旱胁迫。
