Li Shichen, Ahmed Waqar, Jiang Tao, Yang Dehai, Yang Linyuan, Hu Xiaodong, Zhao Meiwei, Peng Xiaoci, Yang Yingfen, Zhang Wei, Li Mingmin, Zhao Zhengxiong
Yunnan Agricultural University, Kunming, Yunnan, 650000, China.
Production Department, Yunnan Hongta Group Dali Cigarette Factory, Dali, Yunnan, 671000, China.
BMC Plant Biol. 2025 Mar 27;25(1):393. doi: 10.1186/s12870-025-06390-4.
Nitrogen (N) is crucial for plant growth and is distributed across various N morphologies within plant organs. However, the mechanisms controlling the distribution of these N morphologies are not fully understood. This study investigated key amino acid (AA) biosynthesis pathways regulating N distribution and their impact on plant physiology and growth.
We examined N distribution in the leaves, stems, and roots of two tobacco cultivars (Hongda and K326) under different N treatments at 75, and 100 days after transplanting (DAT). Transcriptome analysis was performed at 75 and 100 DAT to explore N distribution and AA metabolism pathways. Weighted gene co-expression network analysis (WGCNA) identified pathways regulating N distribution, and the Mantel test assessed the impact of N treatments, growth stages, and cultivars on N distribution.
Statistically significant differences in N distribution were observed across environmental conditions, growth stages, cultivars, and plant organs (p < 0.05). WGCNA identified phenylalanine metabolism (ko00360), alanine, aspartate, and glutamate metabolism (ko00250), and glycine, serine, and threonine metabolism (ko00260) pathways regulating the distribution of N (sodium dodecyl sulfate insoluble N), N (water soluble N), and N (sodium dodecyl sulfate soluble N), respectively. Increased N application promoted N accumulation, while earlier growth stages and cultivar Hongda favored N distribution. N distribution was inhibited under high N conditions. Gene expression in these pathways correlated with N distribution, biomass, and N accumulation.
This study elucidates the mechanisms regulating N distribution in tobacco, emphasizing the role of AA metabolism pathways. These findings are essential for improving N utilization and optimizing N management practices, ultimately enhancing crop productivity and supporting sustainable agricultural practices.
氮（N）对植物生长至关重要，且在植物器官内以多种氮形态分布。然而，控制这些氮形态分布的机制尚未完全明确。本研究调查了调节氮分布的关键氨基酸（AA）生物合成途径及其对植物生理和生长的影响。
我们在移栽后75天和100天（DAT）对两个烟草品种（红大和K326）在不同氮处理下的叶片、茎和根中的氮分布进行了检测。在75天和100天DAT进行转录组分析，以探究氮分布和氨基酸代谢途径。加权基因共表达网络分析（WGCNA）确定了调节氮分布的途径，Mantel检验评估了氮处理、生长阶段和品种对氮分布的影响。
在不同环境条件、生长阶段、品种和植物器官间观察到氮分布存在统计学显著差异（p < 0.05）。WGCNA分别确定了调节氮（十二烷基硫酸钠不溶性氮）、氮（水溶性氮）和氮（十二烷基硫酸钠可溶性氮）分布的苯丙氨酸代谢（ko00360）、丙氨酸、天冬氨酸和谷氨酸代谢（ko00250）以及甘氨酸、丝氨酸和苏氨酸代谢（ko00260）途径。增加施氮量促进了氮积累，而较早的生长阶段和红大品种有利于氮分布。在高氮条件下氮分布受到抑制。这些途径中的基因表达与氮分布、生物量和氮积累相关。
本研究阐明了烟草中调节氮分布的机制，强调了氨基酸代谢途径的作用。这些发现对于提高氮利用率和优化氮管理措施至关重要，最终可提高作物生产力并支持可持续农业实践。
