Li Peng, Tan Xibei, Liu Ruitao, Rahman Faiz Ur, Jiang Jianfu, Sun Lei, Fan Xiucai, Liu Jihong, Liu Chonghuai, Zhang Ying
National Key Laboratory for Germplasm Innovation and Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou 450000, China.
Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430000, China.
Hortic Res. 2023 Apr 2;10(5):uhad063. doi: 10.1093/hr/uhad063. eCollection 2023 May.
Grape white rot, a devastating disease of grapevines caused by (Speg.) Sacc., leads to significant yield losses in grape. Breeding grape cultivars resistant to white rot is essential to reduce the regular use of chemical treatments. In recent years, Chinese grape species have gained more attention for grape breeding due to their high tolerance to various biotic and abiotic factors along with changing climatic conditions. In this study, we employed whole-genome resequencing (WGR) to genotype the parents of 'Manicure Finger' (, female) and '0940' (, male), along with 101 mapping population individuals, thereby constructing a linkage genetic map. The linkage map contained 9337 single-nucleotide polymorphism (SNP) markers with an average marker distance of 0.3 cM. After 3 years of phenotypic evaluation of the progeny for white rot resistance, we confirmed one stable quantitative trait locus (QTL) for white rot resistance on chromosome 3, explaining up to 17.9% of the phenotypic variation. For this locus, we used RNA-seq to detect candidate gene expression and identified as a candidate gene involved in white rot resistance. Finally, we demonstrated that recombinant PR1 protein could inhibit the growth of and that overexpression of in susceptible increased grape resistance to the pathogen.
葡萄白腐病是由（斯佩格）萨卡多引起的一种毁灭性葡萄病害，会导致葡萄产量大幅损失。培育抗白腐病的葡萄品种对于减少化学药剂的常规使用至关重要。近年来，由于中国葡萄品种对各种生物和非生物因素具有高度耐受性，且能适应不断变化的气候条件，因此在葡萄育种中受到了更多关注。在本研究中，我们利用全基因组重测序（WGR）对‘美人指’（♀）和‘0940’（♂）的亲本以及101个作图群体个体进行基因分型，从而构建了一个连锁遗传图谱。该连锁图谱包含9337个单核苷酸多态性（SNP）标记，平均标记间距为0.3厘摩。在对后代进行了3年的白腐病抗性表型评估后，我们在3号染色体上确认了一个稳定的白腐病抗性数量性状位点（QTL），可解释高达17.9%的表型变异。针对该位点，我们利用RNA测序检测候选基因表达，并鉴定出一个参与白腐病抗性的候选基因。最后，我们证明重组PR1蛋白能够抑制病原菌的生长，并且在感病葡萄品种中过表达该基因可提高葡萄对病原菌的抗性。
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