低强度激光对人牙髓干细胞增殖、血管生成及牙本质生成分化的影响

Effect of low-level laser on proliferation, angiogenic and dentinogenic differentiation of human dental pulp stem cells.

作者信息

Rezaei Fatemeh, Shakoori Shahrzad, Fazlyab Mahta, Esnaashari Ehsan, Savadkouhi Sohrab Tour

机构信息

Department of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

出版信息

BMC Oral Health. 2025 Mar 27;25(1):441. doi: 10.1186/s12903-025-05656-5.

DOI:10.1186/s12903-025-05656-5

PMID:40148901

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11948823/

Abstract

BACKGROUND

The aim was to evaluate the effect of single and double doses of low-level laser irradiation on proliferation of human dental pulp stem cells (DPSC) and expression of vascular endothelial growth factor (VEGF) and dentine sialoprotein (DSP).

METHODS

In this experimental in vitro study, after confirming the stemness of DPSCs, the cells were cultured in Dulbecco's Modified Eagle Medium (DMEM) for MTT assay and VEGF-ELISA and osteogenic medium for DSP-ELISA. The wells containing DPSCs were divided into three main groups and 9 subgroups (n = 7). In groups with single low-level laser, 660-nm diode laser was irradiated at 100 mW and 3 J/cm energy density for 15 s. In groups with double doses of low-level laser the second identical irradiation was after 48 h. The MTT-assay and ELISA for DSP/VEGF (dentinogenic/angiogenic differentiation) were performed at 1, 7 and 14 days post irradiation. Using the SPSS software 20 (SPSS, Chicago, Ill, USA) with 95% confidence interval (P = 0.05), a two-way ANOVA test with Tukey's post hoc test was used for the effect of LLLI on VEGF and DSP. The One-Way ANOVA was used for of cell proliferation.

RESULTS

Higher proliferation rate in both single and double low-level laser was reported. The difference was statistically significant for double doses of low-level laser (P = 0.001, P = 0.020 and P = 0.000 for 1, 7 and 14 days, respectively). Also after one, 7 and 14 days, cells in significant increase in DSP (P > 0.05) and VEGF (P > 0.05) was observed that was significantly higher for double doses of low-level laser.

CONCLUSIONS

Low level laser enhanced the mitochondrial activity and proliferation of DPSCs. Increased production of DSP/VEGF indicates dentinogenic/angiogenic activity.

CLINICAL RELEVANCE

Low level laser increases the proliferation of DPSCs, elevates the production of VEGF (which means better angiogenesis in regenerative treatments) and increases the production of DSP (which means better dentinogenesis in vital pulp treatments).

摘要

背景

目的是评估单剂量和双剂量低强度激光照射对人牙髓干细胞（DPSC）增殖以及血管内皮生长因子（VEGF）和牙本质涎蛋白（DSP）表达的影响。

方法

在这项体外实验研究中，确认DPSC的干性后，将细胞培养于杜氏改良 Eagle 培养基（DMEM）中用于MTT 检测和VEGF-ELISA，培养于成骨培养基中用于DSP-ELISA。含有DPSC的孔被分为三个主要组和9个亚组（n = 7）。在单剂量低强度激光组中，用660 nm二极管激光以100 mW和3 J/cm的能量密度照射15秒。在双剂量低强度激光组中，第二次相同照射在48小时后进行。照射后1、7和14天进行MTT检测以及DSP/VEGF（牙本质生成/血管生成分化）的ELISA检测。使用SPSS软件20（SPSS，美国伊利诺伊州芝加哥），置信区间为95%（P = 0.05），采用双向方差分析和Tukey事后检验来分析低强度激光照射对VEGF和DSP的影响。单向方差分析用于分析细胞增殖情况。

结果

单剂量和双剂量低强度激光照射均显示出更高的增殖率。双剂量低强度激光照射的差异具有统计学意义（分别在1、7和14天时P = 0.001、P = 0.020和P = 0.000）。同样，在1、7和14天后，观察到DSP（P > 0.05）和VEGF（P > 0.05）显著增加，双剂量低强度激光照射时增加更为显著。