Moharami Reza, Karimzadeh Zahra, Golsanamlu Zahra, Gharakhani Afshin, Rahimpour Elaheh, Jouyban Abolghasem
Pharmaceutical Analysis Research Center and Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Research Center for Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran.
BMC Chem. 2025 Mar 27;19(1):80. doi: 10.1186/s13065-025-01456-8.
According to the narrow therapeutic range and multiple adverse effects of cyclosporine and the need for its therapeutic drug monitoring (TDM), in this study, an efficient zeolitic imidazolate framework-8 metal-organic framework (ZIF-8 MOF) based nanoprobe was designed for simple, rapid and high sensitive its quantification in plasma samples. After the successful synthesis of the ZIF-8 MOF, under the optimum condition, the fluorescence emission of ZIF-8 MOF, measured at an excitation wavelength of 370 nm and an emission wavelength of 417 nm, was enhanced with increasing cyclosporine concentration, due to the specific interactions between cyclosporine and the nanoprobe, including hydrogen bonding and hydrophobic effects. The nanoprobe showed a linear correlation between the analytical response and cyclosporine concentration in the concentration range of 0.01-1.0 µg mL, with a detection limit of 0.003 µg mL. Acceptable precision was achieved, evidenced by intra-day and inter-day relative standard deviations of 0.4% and 0.5%, respectively. Recovery between 97.1% and 102.1% in plasma samples indicated the method's reliability in practical applications.
鉴于环孢素治疗窗窄、不良反应多以及治疗药物监测（TDM）的需求，本研究设计了一种基于高效沸石咪唑酯骨架-8金属有机框架（ZIF-8 MOF）的纳米探针，用于简单、快速且高灵敏地定量血浆样本中的环孢素。成功合成ZIF-8 MOF后，在最佳条件下，由于环孢素与纳米探针之间存在包括氢键和疏水作用在内的特异性相互作用，在370 nm激发波长和417 nm发射波长下测得的ZIF-8 MOF荧光发射随环孢素浓度增加而增强。该纳米探针在0.01 - 1.0 μg/mL浓度范围内，分析响应与环孢素浓度呈线性相关，检测限为0.003 μg/mL。日内和日间相对标准偏差分别为0.4%和0.5%，表明该方法具有可接受的精密度。血浆样本回收率在97.1%至102.1%之间，表明该方法在实际应用中具有可靠性。
