• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

S-亚硝基谷胱甘肽不是有机阴离子转运多肽1B1（OATP1B1）的底物，但能刺激其表达和活性。

S-Nitrosoglutathione Is Not a Substrate of OATP1B1, but Stimulates Its Expression and Activity.

作者信息

Abalenikhina Yulia V, Shchulkin Aleksey V, Suchkova Olga N, Ananyeva Pelageya D, Mylnikov Pavel Yu, Yakusheva Elena N, Suchkov Igor A, Kalinin Roman E

机构信息

Department of Biological Chemistry, Ryazan State Medical University, 390026 Ryazan, Russia.

Department of Pharmacology, Ryazan State Medical University, 390026 Ryazan, Russia.

出版信息

Biomolecules. 2025 Mar 17;15(3):428. doi: 10.3390/biom15030428.

DOI:10.3390/biom15030428
PMID:40149964
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11940419/
Abstract

S-nitrosoglutathione (GSNO) is the S-nitrosated derivative of glutathione (GSH). GSNO is an endogenous class of NO donors and a natural NO depot in biological systems. Organic anion transporting polypeptide 1B1 (OATP1B1) is an influx transporter that is expressed in the liver. OATP1B1 plays an important role in the transport of endogenous and exogenous substances. Various pathways for the regulation of OATP1B1 have been described. In the present study, the involvement of OATP1B1 in GSNO transport and the regulation of OATP1B1 by GSNO was examined. For HEK293-OATP1B1, it has been shown that GSNO is not a substrate of OATP1B1, but OATP1B1 can participate in the transport of GSH across the cell membrane. GSNO at concentrations of 1-100 μM and exposure for 3 h do not affect the expression and activity of OATP1B1, but exposure for 24 and 72 h stimulates the expression of the gene, OATP1B1, and transporter activity. Up-regulation of OATP1B1 by GSNO is carried out through the NO-cGMP signaling pathway, Nrf2, and LXR.

摘要

S-亚硝基谷胱甘肽（GSNO）是谷胱甘肽（GSH）的S-亚硝化衍生物。GSNO是一类内源性一氧化氮供体，也是生物系统中的天然一氧化氮储存库。有机阴离子转运多肽1B1（OATP1B1）是一种在肝脏中表达的摄取转运体。OATP1B1在内外源性物质的转运中起重要作用。已有多种调节OATP1B1的途径被描述。在本研究中，检测了OATP1B1在GSNO转运中的作用以及GSNO对OATP1B1的调节。对于HEK293-OATP1B1，已表明GSNO不是OATP1B1的底物，但OATP1B1可参与谷胱甘肽跨细胞膜的转运。浓度为1-100μM的GSNO作用3小时不影响OATP1B1的表达和活性，但作用24小时和72小时可刺激OATP1B1基因的表达及转运体活性。GSNO对OATP1B1的上调是通过NO-cGMP信号通路、Nrf2和LXR实现的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/d9c003f8a286/biomolecules-15-00428-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/dbf728a962f8/biomolecules-15-00428-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/838b7334f0e6/biomolecules-15-00428-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/a53c7c212585/biomolecules-15-00428-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/9db3675a70c5/biomolecules-15-00428-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/42b3526713fe/biomolecules-15-00428-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/9fa4e7a031a7/biomolecules-15-00428-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/fc8b0c61d6ca/biomolecules-15-00428-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/e7eb1cd6e52f/biomolecules-15-00428-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/f29d3f7bf77b/biomolecules-15-00428-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/c68d5c1ee8b8/biomolecules-15-00428-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/f2d2ece744ed/biomolecules-15-00428-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/d9c003f8a286/biomolecules-15-00428-g012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/dbf728a962f8/biomolecules-15-00428-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/838b7334f0e6/biomolecules-15-00428-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/a53c7c212585/biomolecules-15-00428-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/9db3675a70c5/biomolecules-15-00428-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/42b3526713fe/biomolecules-15-00428-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/9fa4e7a031a7/biomolecules-15-00428-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/fc8b0c61d6ca/biomolecules-15-00428-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/e7eb1cd6e52f/biomolecules-15-00428-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/f29d3f7bf77b/biomolecules-15-00428-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/c68d5c1ee8b8/biomolecules-15-00428-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/f2d2ece744ed/biomolecules-15-00428-g011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e418/11940419/d9c003f8a286/biomolecules-15-00428-g012.jpg

相似文献

1
S-Nitrosoglutathione Is Not a Substrate of OATP1B1, but Stimulates Its Expression and Activity.S-亚硝基谷胱甘肽不是有机阴离子转运多肽1B1（OATP1B1）的底物，但能刺激其表达和活性。
Biomolecules. 2025 Mar 17;15(3):428. doi: 10.3390/biom15030428.
2
The inducing effect of S-nitrosoglutathione on the expression and activity of organic anion transporting polypeptide 1B1 (OATP1B1) in HepG2 cells.S-亚硝基谷胱甘肽对HepG2细胞中有机阴离子转运多肽1B1（OATP1B1）表达及活性的诱导作用。
Biomed Khim. 2025 Feb;71(1):29-36. doi: 10.18097/PBMCR1487.
3
Downregulation of Organic Anion Transporting Polypeptide (OATP) 1B1 Transport Function by Lysosomotropic Drug Chloroquine: Implication in OATP-Mediated Drug-Drug Interactions.溶酶体亲和性药物氯喹对有机阴离子转运多肽（OATP）1B1转运功能的下调作用：对OATP介导的药物相互作用的影响
Mol Pharm. 2016 Mar 7;13(3):839-51. doi: 10.1021/acs.molpharmaceut.5b00763. Epub 2016 Feb 1.
4
Regulation of organic anion transporting polypeptide 1B1 transport function by concurrent phosphorylation and lysine-acetylation: A novel posttranslational regulation mechanism.通过同时磷酸化和赖氨酸乙酰化对有机阴离子转运多肽1B1转运功能的调控：一种新的翻译后调控机制。
Mol Pharmacol. 2025 Feb;107(2):100007. doi: 10.1016/j.molpha.2024.100007. Epub 2024 Dec 12.
5
Generation of a Cell Line Selectively Producing Functionally Active OATP1B1 Transporter.生成一株选择性产生功能活跃的 OATP1B1 转运体的细胞系。
Biochemistry (Mosc). 2023 Sep;88(9):1267-1273. doi: 10.1134/S0006297923090067.
6
Organic anion transporting polypeptide 1B1: a genetically polymorphic transporter of major importance for hepatic drug uptake.有机阴离子转运多肽 1B1：一种遗传多态性转运体，对肝脏药物摄取具有重要意义。
Pharmacol Rev. 2011 Mar;63(1):157-81. doi: 10.1124/pr.110.002857. Epub 2011 Jan 18.
7
The Effect of Single Nucleotide Variations in the Transmembrane Domain of OATP1B1 on in vitro Functionality.单核苷酸变异对 OATP1B1 跨膜结构域在体外功能的影响。
Pharm Res. 2021 Oct;38(10):1663-1675. doi: 10.1007/s11095-021-03107-8. Epub 2021 Oct 13.
8
OATP1B3 Expression and Function is Modulated by Coexpression with OCT1, OATP1B1, and NTCP.OATP1B3 的表达和功能受与 OCT1、OATP1B1 和 NTCP 共表达的调节。
Drug Metab Dispos. 2020 Aug;48(8):622-630. doi: 10.1124/dmd.119.089847. Epub 2020 Jun 1.
9
Functional Characterization of Six (OATP1B1) Variants Observed in Finnish Individuals with a Psychotic Disorder.六种（OATP1B1）变体在芬兰精神障碍个体中的功能特征分析。
Mol Pharm. 2023 Mar 6;20(3):1500-1508. doi: 10.1021/acs.molpharmaceut.2c00715. Epub 2023 Feb 13.
10
Molecular Mechanisms for the Selective Transport of Dichlorofluorescein by Human Organic Anion Transporting Polypeptide 1B1.二氯荧光素经人有机阴离子转运多肽 1B1 的选择性转运的分子机制。
Drug Metab Dispos. 2024 Oct 16;52(11):1323-1331. doi: 10.1124/dmd.124.001853.

引用本文的文献

1
Molecular and Biochemical Mechanisms of Cardiomyopathy Development Following Prenatal Hypoxia-Focus on the NO System.产前缺氧后心肌病发展的分子和生化机制——聚焦于一氧化氮系统
Antioxidants (Basel). 2025 Jun 16;14(6):743. doi: 10.3390/antiox14060743.

本文引用的文献

1
Generation of a Cell Line Selectively Producing Functionally Active OATP1B1 Transporter.生成一株选择性产生功能活跃的 OATP1B1 转运体的细胞系。
Biochemistry (Mosc). 2023 Sep;88(9):1267-1273. doi: 10.1134/S0006297923090067.
2
Nitric oxide signaling in health and disease.一氧化氮信号在健康和疾病中的作用。
Cell. 2022 Aug 4;185(16):2853-2878. doi: 10.1016/j.cell.2022.06.010.
3
The Interplay between cGMP and Calcium Signaling in Alzheimer's Disease.环鸟苷酸与钙信号在阿尔茨海默病中的相互作用。
Int J Mol Sci. 2022 Jun 24;23(13):7048. doi: 10.3390/ijms23137048.
4
Buffer concentration dramatically affects the stability of S-nitrosothiols in aqueous solutions.缓冲液浓度会极大地影响硫代亚硝酸盐在水溶液中的稳定性。
Nitric Oxide. 2022 Jan 1;118:59-65. doi: 10.1016/j.niox.2021.11.002. Epub 2021 Nov 27.
5
Mechanisms of nitric oxide generation in living systems.生物系统中一氧化氮生成的机制。
Nitric Oxide. 2022 Jan 1;118:1-16. doi: 10.1016/j.niox.2021.10.003. Epub 2021 Oct 21.
6
Endogenous Biomarkers for SLC Transporter-Mediated Drug-Drug Interaction Evaluation.内源性生物标志物用于 SLC 转运体介导的药物相互作用评价。
Molecules. 2021 Sep 10;26(18):5500. doi: 10.3390/molecules26185500.
7
Regulation of B cell functions by S-nitrosoglutathione in the EAE model.通过 EAE 模型中 S-亚硝基谷胱甘肽对 B 细胞功能的调节。
Redox Biol. 2021 Sep;45:102053. doi: 10.1016/j.redox.2021.102053. Epub 2021 Jun 23.
8
Advanced nitric oxide donors: chemical structure of NO drugs, NO nanomedicines and biomedical applications.先进的一氧化氮供体：一氧化氮药物、一氧化氮纳米药物的化学结构及生物医学应用
Nanoscale. 2021 Jan 21;13(2):444-459. doi: 10.1039/d0nr07484e.
9
Functional implications of vascular endothelium in regulation of endothelial nitric oxide synthesis to control blood pressure and cardiac functions.血管内皮在调节内皮型一氧化氮合酶合成以控制血压和心脏功能方面的功能意义。
Life Sci. 2020 Oct 15;259:118377. doi: 10.1016/j.lfs.2020.118377. Epub 2020 Sep 6.
10
Nitric oxide detection methods and .一氧化氮检测方法与…… （原文不完整）
Med Gas Res. 2019 Oct-Dec;9(4):192-207. doi: 10.4103/2045-9912.273957.