Wei Yuyu, Zhang Jingwen, Zhang Yiming, Liu Dingkuo, You Chunxue, Zhang Wenjuan, Ren Chaoqi, Zhao Xin, Li Liu'an, Yu Xiaoxue
Key Laboratory of Intelligent Breeding, Ministry of Agriculture and Rural Affairs (Ministry-Province Joint Establishment), Tianjin Key Laboratory of Agricultural Animal Breeding and Health Breeding, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300392, China.
Tianjin Key Laboratory of Biological Feed Additive Enterprise, S&E Burgeoning Biotechnology (Tianjin) Co., Ltd., Tianjin 300383, China.
Animals (Basel). 2025 Mar 20;15(6):886. doi: 10.3390/ani15060886.
This study aimed to investigate the effects of different supplemental amounts of dandelion flavonoid extracts (DFE) in diets on nutrients in chicken, slaughtering performance, blood biochemical indexes and antioxidant capacity of spent laying hens. A total of 180 560-day-old spent Hy-Line Brown laying hens were randomly divided into five groups. The control group was fed the basal diet, while the experimental groups were supplemented with DFE at levels of 1000, 2000, 4000, and 8000 mg/kg (as T1, T2, T3, and T4 group) in the basal diet, respectively. The variables measured included the content of dandelion flavonoids in layer hen thigh meat and breast meat, slaughter performance, blood biochemical indexes, and antioxidant capacity. Data were subjected to a one-way analysis of variance (one-way ANOVA) to assess the impact of DFE supplementation compared to the control group on study outcomes. The results showed that dietary supplementation with DFE can increase the content of dandelion flavonoids in layer hen meat. The contents of rutin in layer hen breast meat of groups T1, T2, T3, and T4 were 1.37, 4.41, 16.26, and 36.03 ng/g, respectively, and the contents of quercetin was 2.58, 1.36, 4.98, 12.48 ng/g. In layer hen thigh meat of groups T1, T2, T3, and T4, the contents of rutin were 11.48, 15.98, 44.43, 122.32 ng/g, and the contents of quercetin were 9.96, 13.14, 23.15, 38.09 ng/g, respectively. The addition of DFE increased the total phenol content of the feed and highly significantly elevated the total phenol content of layer hen meat ( < 0.01), and the total phenol content of chicken meat was strongly and positively correlated with the total phenol content of the feed. DFE supplementation significantly decreased abdominal fat percentage ( < 0.05) and increased crude fat content in chicken ( < 0.05). The addition of DFE reduced aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities ( < 0.05), decreased triglyceride (TG), total cholesterol (TC), low-density lipoprotein (LDL) cholesterol (LDL-C), glucose (GLU), and malondialdehyde (MDA) contents ( < 0.05), and increased the content of albumin (ALB), total antioxidant (T-AOC) capacity and total superoxide dismutase (T-SOD), glutathione peroxidase (GSH-Px) activity ( < 0.05). Dietary supplementation of DFE at different concentrations could significantly increase the content of dandelion flavonoids in the muscle of spent laying hens, reduce the abdominal fat rate in hens, effectively reduce blood lipid levels, effectively increase crude fat content in thigh muscle, and enhance the body's antioxidant capacity and liver function.
本研究旨在探讨日粮中添加不同量蒲公英黄酮提取物（DFE）对蛋鸡营养物质、屠宰性能、血液生化指标及抗氧化能力的影响。选取180只560日龄海兰褐商品蛋鸡，随机分为五组。对照组饲喂基础日粮，试验组在基础日粮中分别添加1000、2000、4000和8000 mg/kg的DFE（分别作为T1、T2、T3和T4组）。测定指标包括蛋鸡大腿肉和胸肉中蒲公英黄酮含量、屠宰性能、血液生化指标及抗氧化能力。采用单因素方差分析评估与对照组相比，添加DFE对研究结果的影响。结果表明，日粮中添加DFE可提高蛋鸡肉中蒲公英黄酮含量。T1、T2、T3和T4组蛋鸡胸肉中芦丁含量分别为1.37、4.41、16.26和36.03 ng/g，槲皮素含量分别为2.58、1.36、4.98和12.48 ng/g。T1、T2、T3和T4组蛋鸡大腿肉中芦丁含量分别为11.48、15.98、44.43和122.32 ng/g，槲皮素含量分别为9.96、13.14、23.15和38.09 ng/g。添加DFE提高了饲料中总酚含量，并极显著提高了蛋鸡肉中总酚含量（P<0.01），鸡肉中总酚含量与饲料中总酚含量呈强正相关。添加DFE显著降低了腹脂率（P<0.05），提高了鸡肉中粗脂肪含量（P<0.05）。添加DFE降低了天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）活性（P<0.05），降低了甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）、葡萄糖（GLU）和丙二醛（MDA）含量（P<0.05），提高了白蛋白（ALB）含量、总抗氧化能力（T-AOC）和总超氧化物歧化酶（T-SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性（P<0.05）。日粮中添加不同浓度的DFE可显著提高商品蛋鸡肌肉中蒲公英黄酮含量，降低母鸡腹脂率，有效降低血脂水平，有效提高大腿肌肉中粗脂肪含量，增强机体抗氧化能力和肝功能。
