基于后生元的口腔崩解膜预防口腔微生物群失调的研究进展

Development of a Postbiotic-Based Orodispersible Film to Prevent Dysbiosis in the Oral Cavity.

作者信息

Rebelo Mariana B, Oliveira Cláudia S, Tavaria Freni K

机构信息

CBQF-Centre for Biotechnology and Fine Chemistry-Associated Laboratory, Portuguese Catholic University, 4169-005 Porto, Portugal.

出版信息

Front Biosci (Elite Ed). 2025 Mar 24;17(1):26987. doi: 10.31083/FBE26987.

DOI:10.31083/FBE26987

PMID:40150984

Abstract

BACKGROUND

Oral diseases affect over three billion peopleand are among the most commonly observed infections worldwide. Recent studies have shown that controlling the ecology of the oralome is more effective in reducing the risk of caries than the complete removal of both harmful and beneficial microorganisms. This work aimed to develop a strategy for preventing dysbiosis in the oral cavity by applying a postbiotic-based orodispersible film.

METHODS

226V and L26 were cultured in De Man-Rogosa-Sharpe (MRS) broth for 48 hours, followed by centrifugation and filtration. Then, the resultant postbiotics were then subjected to various dilutions (10% (v/v), 20% (v/v), 40% (v/v), 60% (v/v) and 100% (v/v)) and co-incubated with Antimicrobial efficacy, minimal inhibitory concentration, the time required to inhibit growth, and antibiofilm properties of the postbiotics were assessed. Subsequently, an orodispersible film comprising polymers and plasticizers, namely Xanthan gum, maltodextrin, and glycerol, was developed as a vehicle for postbiotic delivery. Formulation optimization, physical property evaluation, and cytotoxicity against the TR146 human oral cell line (TR146 cell line) were conducted.

RESULTS

Postbiotics demonstrated antimicrobial and antibiofilm activity against following 24-hour co-incubation. The minimal inhibitory concentration for combined postbiotics administration was 20% (v/v). Remarkably, 79.6 ± 8.15% inhibition of biofilm formation was achieved using 100% (v/v) of the postbiotic derived from 226V. Incorporating postbiotics did not compromise the dissolution time of orodispersible films, all exceeding 20 minutes. Furthermore, solubility improved following postbiotic addition, facilitating ease of handling. Importantly, postbiotic-impregnated orodispersible films were non-cytotoxic when exposed to the TR146 cell line.

CONCLUSIONS

These findings underscore the potential of orodispersible films loaded with postbiotics as a promising potential intervention for oral dysbiosis.

摘要

背景

口腔疾病影响着超过30亿人，是全球最常见的感染之一。最近的研究表明，控制口腔微生物群落生态在降低龋齿风险方面比完全清除有害和有益微生物更有效。这项工作旨在通过应用基于后生元的口腔崩解膜来制定一种预防口腔生态失调的策略。

方法

将226V和L26在德氏改良罗氏培养基（MRS肉汤）中培养48小时，然后进行离心和过滤。然后，将所得的后生元进行各种稀释（10%（v/v）、20%（v/v）、40%（v/v）、60%（v/v）和100%（v/v）），并与进行共培养。评估了后生元的抗菌效果、最小抑菌浓度、抑制生长所需时间和抗生物膜特性。随后，开发了一种包含聚合物和增塑剂（即黄原胶、麦芽糊精和甘油）的口腔崩解膜，作为后生元递送的载体。进行了配方优化、物理性质评估以及对TR146人口腔细胞系（TR146细胞系）的细胞毒性测试。

结果

后生元在共培养24小时后表现出抗菌和抗生物膜活性。联合使用后生元的最小抑菌浓度为20%（v/v）。值得注意的是，使用100%（v/v）源自226V的后生元可实现79.6±8.15%的生物膜形成抑制率。加入后生元并未影响口腔崩解膜的溶解时间，所有溶解时间均超过20分钟。此外，添加后生元后溶解度提高，便于处理。重要的是，后生元浸渍的口腔崩解膜在接触TR146细胞系时无细胞毒性。