Zubkov A V, Butova L G, Kuzmina N S, Zubkova I V, Fadeev V V, Svitich O A
I. Mechnikov Research Institute of Vaccine and Sera, Moscow, Russia.
I. M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia.
Bull Exp Biol Med. 2025 Feb;178(4):431-436. doi: 10.1007/s10517-025-06351-9. Epub 2025 Mar 28.
Fragments of the thyrotropin receptor gene (TSHR) encoding 432- and 389-bp regions of the extracellular domain corresponding to the autoantibody binding sites in autoimmune diseases of the thyroid gland (Graves' disease and Hashimoto's thyroiditis, respectively) were cloned based on mRNA isolated not only from the thyroid tissue, but also from the whole blood of patients with toxic diffuse goiter (Graves' disease). In a group of 18 patients, the 432-bp fragment was detected in 6 patients and the 389-bp fragment was detected in 3 patients. In one patient, both fragments were isolated from the blood. The results indicate that TSHR is expressed in the blood in 30% of patients with Graves' disease.
基于不仅从甲状腺组织，而且从毒性弥漫性甲状腺肿（格雷夫斯病）患者的全血中分离出的mRNA，克隆了促甲状腺激素受体基因（TSHR）的片段，该片段编码甲状腺自身免疫性疾病（分别为格雷夫斯病和桥本甲状腺炎）中与自身抗体结合位点相对应的细胞外结构域的432bp和389bp区域。在一组18名患者中，6名患者检测到432bp片段，3名患者检测到389bp片段。在一名患者中，两个片段均从血液中分离出来。结果表明，30%的格雷夫斯病患者血液中表达促甲状腺激素受体。
