Rinkevich Baruch, Pomponi Shirley A
Israel Oceanographic & Limnological Research, National Institute of Oceanography, Tel Shikmona, 3108001, Haifa, Israel.
Florida Atlantic University, Harbor Branch Oceanographic Institute, Fort Pierce, FL, USA.
In Vitro Cell Dev Biol Anim. 2025 Mar 28. doi: 10.1007/s11626-025-01029-y.
Although cell cultures from marine invertebrates have great potential as valuable tools in various scientific fields, nearly all attempts to culture these cells in vitro have consistently failed, and the reasons for this remain unclear. The ongoing failure to develop stable, long-term cell cultures from marine invertebrates, despite varied species and methods employed, highlights significant knowledge gaps in understanding their in vitro requirements. These gaps impede progress, underscoring the complexity of marine invertebrate cells and the need for innovative approaches to overcome challenges in the field. When reviewing recent literature on the key data deficiencies and challenges behind the failure to develop marine invertebrate cell cultures, we identified and discussed four major knowledge gaps: (1) optimizing culture media, (2) strategies to extend stemness of isolated cells, (3) using "omics" to enhance cell culture, and (4) selecting suitable cell types for in vitro cultures. Bridging these gaps is crucial for advancing marine invertebrate cell culture systems. Yet, given the current state-of-the-art, addressing these gaps and advancing the discipline necessitate comprehensive, integrated, and species- or cell-specific strategies, along with close collaboration among laboratories working on diverse species.
尽管来自海洋无脊椎动物的细胞培养物作为各种科学领域中有价值的工具具有巨大潜力，但几乎所有在体外培养这些细胞的尝试都一直失败，其原因仍不清楚。尽管采用了不同的物种和方法，但仍未能从海洋无脊椎动物中开发出稳定的长期细胞培养物，这凸显了在了解其体外需求方面存在重大知识空白。这些空白阻碍了进展，强调了海洋无脊椎动物细胞的复杂性以及需要创新方法来克服该领域的挑战。在回顾有关未能开发海洋无脊椎动物细胞培养物背后的关键数据缺陷和挑战的近期文献时，我们确定并讨论了四个主要知识空白：（1）优化培养基，（2）延长分离细胞干性的策略，（3）利用“组学”增强细胞培养，以及（4）选择适合体外培养的细胞类型。弥合这些差距对于推进海洋无脊椎动物细胞培养系统至关重要。然而，鉴于当前的技术水平，解决这些差距并推动该学科发展需要全面、综合且针对物种或细胞的策略，以及研究不同物种的实验室之间的密切合作。
