Sbissi Imed, Dali Hana, Hamra Rihab, Boujemaa Emeni, Nasr Mohamed Aziz Haj, Ghodhbane-Gtari Faten, Gtari Maher
Institute of Arid Lands of Medenine, LR Pastoral Ecosystems and Promotion of Spontaneous Plants and Associated Microorganisms, University of Gabes, Medenine, Tunisia.
Department of Biological and Chemical Engineering, USCR Molecular Bacteriology and Genomics, National Institute of Applied Sciences and Technology, University of Carthage, Tunis Cedex 2080, Tunisia.
Int J Syst Evol Microbiol. 2025 Mar;75(3). doi: 10.1099/ijsem.0.006733.
Phylogenetic analysis of the genus , utilizing both 16S rRNA gene sequences and comprehensive whole-genome data, revealed its polyphyletic nature, necessitating a revision to accommodate phylogenetically distinct species. Based on established threshold values for genus demarcation - specifically, 16S rRNA gene similarity, Average Amino Acid Identity and Percentage of Conserved Proteins - a notably substantial divergence was observed within the genus , and the division of into four distinct genera is strongly supported. To address this, we propose the establishment of four new genera: gen. nov., gen. nov., gen. nov. and gen. nov. These classifications accommodate species that are significantly divergent from the type species of , thereby more accurately reflecting their distinct evolutionary lineages. Additionally, is proposed to be reclassified within the genus as comb. nov. based on phylogenetic evidence indicating a closer evolutionary relationship to species than to other members of . Our analysis, which included assessments of 16S rRNA gene similarity, Average Nucleotide Identity, and digital DNA-DNA hybridization values exceeding species delineation thresholds, further supports the reclassification of Jiang . 2024 as a later heterotypic synonym of Du . 2023.
利用16S rRNA基因序列和全面的全基因组数据对该属进行系统发育分析，揭示了其多系性质，因此有必要进行修订以容纳系统发育上不同的物种。基于已确立的属划分阈值——具体而言，16S rRNA基因相似性、平均氨基酸同一性和保守蛋白百分比——在该属内观察到显著的差异，强烈支持将其划分为四个不同的属。为解决此问题，我们提议建立四个新属：新属、新属、新属和新属。这些分类容纳了与该属模式种有显著差异的物种，从而更准确地反映了它们不同的进化谱系。此外，基于系统发育证据表明与物种的进化关系比与该属其他成员更密切，提议将重新分类为属内的新组合。我们的分析包括对16S rRNA基因相似性、平均核苷酸同一性和超过物种划分阈值的数字DNA-DNA杂交值的评估，进一步支持将Jiang. 2024重新分类为Du. 2023的晚出异名。
