College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2020 May;70(5):3123-3133. doi: 10.1099/ijsem.0.004141.
Chiou . 2018 and McFrederick . 2018 are closely related, and they share 100 % 16S rRNA gene sequence similarity, 99.6 % gene sequence similarity, 100 % gene sequence similarity, 97.3 % average nucleotide identity (ANI) value and 76.6 % DNA-DNA hybridization (DDH) value, indicating that they represent the same species. Fatty acid methyl esters (FAME) analysis and phenotypic characterization also indicated that and are very similar. We propose Chiou . 2018 as a later heterotypic synonym of McFrederick . 2018. The taxonomic position of subsp. in the group was re-examined using a polyphasic approach, including sequence analyses of 16S rRNA, , and genes, average nucleotide identity analysis, DNA-DNA hybridization, fatty acid methyl ester analysis and phenotypic characterization. Results of 16S rRNA gene sequence analysis indicated that subsp. was closely related to subsp. , and in the group, sharing 99.6-99.7 % 16S rRNA gene sequence similarities. Results of , and gene sequence analyses indicated that subsp. was most closely related to subsp. , having 91.8 % gene sequence similarity, 98.9 % gene sequence similarity and 93.1 % gene sequence similarity. subsp. DSM 16365 shared 95.6 % ANI value and 62.9 % DDH value with subsp. ATCC 14917. The low DDH value confirmed that subsp. and subsp. represent two different species, rather than two different subspecies in the group. On the basis of the data from polyphasic characterization obtained in the present study and in previous studies, subsp. is elevated to the species level and represents a novel species of the genus , for which the name sp. nov. is proposed and the type strain is DKO 22 (=CIP 108320=DSM 16365=JCM 16169). Two novel Gram-stain-positive bacterial strains, designated 1206-1 and F027-1-2, were isolated from traditional pickle in Heilongjiang Province, PR China, and from the intestinal tract of a honey bee () in Hubei Province, PR China, respectively. The two bacteria were characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, fatty acid methyl ester analysis, average nucleotide identity analysis, DNA-DNA hybridization analysis and an analysis of phenotypic features. The results of 16S rRNA gene sequence analysis indicated that strains 1206-1 and F027-1-2 were distantly related to , , , , , , , , , and . Strain 1206-1 exhibited 94.2-96.4 % 16S rRNA gene sequence similarities, 69.5-83.3 % gene sequence similarities and 73.1-90.3 % gene sequence similarities to type strains of phylogenetically related species. ANI and DDH values between strain 1206-1 and the type strains of phylogenetically related species were 52.7-73.7 % and 21.1-30.1 %, respectively. On the basis of the data obtained in the present study, a novel species, sp. nov. is proposed and the type strain is 1206-1 (=CCM 8981=CCTCC AB 2019200=LMG 31620).
邱等。2018 年和麦克弗雷德里克等。2018 年密切相关，它们的 16S rRNA 基因序列相似度为 100%，基因序列相似度为 99.6%，基因序列相似度为 100%，平均核苷酸同一性（ANI）值为 97.3%，DNA-DNA 杂交（DDH）值为 76.6%，表明它们代表同一物种。脂肪酸甲酯（FAME）分析和表型特征也表明和非常相似。我们提议将邱等。2018 年作为麦克弗雷德里克等。2018 年的后来的异型同义词。使用多相方法重新检查了亚种在群中的分类地位，包括 16S rRNA、、和基因序列分析、平均核苷酸同一性分析、DNA-DNA 杂交、脂肪酸甲酯分析和表型特征。16S rRNA 基因序列分析结果表明，亚种与群中的亚种、和密切相关，16S rRNA 基因序列相似度为 99.6-99.7%。和基因序列分析结果表明，亚种与亚种最为密切相关，基因序列相似度为 91.8%，基因序列相似度为 98.9%，基因序列相似度为 93.1%。DSM 16365 与亚种 ATCC 14917 共享 95.6%的 ANI 值和 62.9%的 DDH 值。低 DDH 值证实亚种和亚种代表两个不同的物种，而不是群中的两个不同亚种。基于本研究和以往研究中多相特征获得的数据，亚种被提升到物种水平，代表了一个新的属物种，提议使用新种名 sp. nov.，其模式菌株为 DKO 22（=CIP 108320=DSM 16365=JCM 16169）。从中国黑龙江省传统泡菜和中国湖北省一只蜜蜂（）肠道中分别分离到两株革兰氏阳性细菌菌株，分别命名为 1206-1 和 F027-1-2。采用多相方法对这两株细菌进行了鉴定，包括 16S rRNA 基因序列分析、基因序列分析、基因序列分析、脂肪酸甲酯分析、平均核苷酸同一性分析、DNA-DNA 杂交分析和表型特征分析。16S rRNA 基因序列分析结果表明，菌株 1206-1 和 F027-1-2与、、、、、、、、和亲缘关系较远。菌株 1206-1 与系统发育上相关的种的 16S rRNA 基因序列相似度为 94.2-96.4%，与基因序列相似度为 69.5-83.3%，与基因序列相似度为 73.1-90.3%。菌株 1206-1 与系统发育上相关的种的 ANI 和 DDH 值分别为 52.7-73.7%和 21.1-30.1%。基于本研究获得的数据，提出了一个新种，命名为 sp. nov.，其模式菌株为 1206-1（=CCM 8981=CCTCC AB 2019200=LMG 31620）。
