小鼠脑内空间代谢组学与同位素示踪实验方案。

Protocol for spatial metabolomics and isotope tracing in the mouse brain.

作者信息

Sontising Watit, Yanchik-Slade Francine, Rodriguez-Navas Carlos, Hossen Md Amir, Gonzalez-Rodriguez Manuel, Vaynshteyn Jake, Yamaguchi Shinichi, Boutaghou Mohamed Nazim, Marin-Valencia Isaac

机构信息

The Abimael Laboratory of Neurometabolism, Department of Neurology, Icahn School of Medicine at Mount Sinai, New York, NY, USA.

Shimadzu Scientific Instruments, Columbia, MD, USA.

出版信息

STAR Protoc. 2025 Mar 27;6(2):103716. doi: 10.1016/j.xpro.2025.103716.

DOI:10.1016/j.xpro.2025.103716

PMID:40153438

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11995059/

Abstract

Mass spectrometry imaging enables high-resolution spatial chemical mapping, yet its application for dynamic analysis with tracers poses challenges. Here, we present a protocol for spatial metabolomics and isotope tracing in the mouse brain. We describe steps for tracer administration, tissue collection, and cryosectioning. We then detail procedures for matrix application, ion identification, and data analysis. This protocol delivers high-quality spatial metabolomics data and is well suited for region-specific tracing analysis in the brain.

摘要

质谱成像能够实现高分辨率的空间化学图谱绘制，但其在使用示踪剂进行动态分析方面存在挑战。在此，我们介绍一种用于小鼠大脑空间代谢组学和同位素示踪的方案。我们描述了示踪剂给药、组织采集和冷冻切片的步骤。然后，我们详细说明了基质应用、离子鉴定和数据分析的程序。该方案可提供高质量的空间代谢组学数据，非常适合大脑中特定区域的示踪分析。