红细胞凝集素免疫试验（Erythro-LIT）在神经囊尾蚴病免疫诊断中的应用

Erythro-lectin immuno test (Erythro-LIT) in the immunodiagnosis of neurocysticercosis.

Rossi C L, Prigenzi L S, Livramento J A

Departamento de Patologia Clínica, Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Brasil.

Braz J Med Biol Res. 1989;22(1):69-75.

Abstract

The effectiveness of the Erythro-Lectin Immuno Test (Erythro-LIT) to detect anticysticercus antibodies was tested using cerebrospinal fluid (CFS) from patients with neurocysticercosis. 2. Both Erythro-LIT and complement fixation (CF) were used to detect antibodies in 36 CSF samples from cysticercotic patients. Erythro-LIT detected anticysticercus antibodies in 35 CSF samples (97%) and CF in 26 (72%). The antibody titers ranged from 1:4 to 1:4096 for Erythro-LIT (mean geometric titer = 282.65) and 1:1 to 1:64 in CF (mean geometric titer = 7.92). 3. When greater than or equal to 1:16 Erythro-LIT titer was used as a significant diagnostic cut-off value, the sensitivity and specificity of Erythro-LIT were 92% and 100%, respectively, for CFS. 4. The high sensitivity and specificity demonstrated here, together with the simplicity and low cost of the test, make the Erythro-LIT a potentially useful method for screening for specific antibodies in neurocysticercosis.

摘要

使用来自神经囊尾蚴病患者的脑脊液（CFS），对红细胞凝集素免疫试验（Erythro-LIT）检测抗囊尾蚴抗体的有效性进行了测试。2. 采用红细胞凝集素免疫试验（Erythro-LIT）和补体结合试验（CF）对36份囊尾蚴病患者的脑脊液样本中的抗体进行检测。红细胞凝集素免疫试验（Erythro-LIT）在35份脑脊液样本（97%）中检测到抗囊尾蚴抗体，补体结合试验（CF）在26份样本（72%）中检测到抗体。红细胞凝集素免疫试验（Erythro-LIT）的抗体滴度范围为1:4至1:4096（平均几何滴度 = 282.65），补体结合试验（CF）的抗体滴度范围为1:1至1:64（平均几何滴度 = 7.92）。3. 当红细胞凝集素免疫试验（Erythro-LIT）滴度大于或等于1:16被用作显著诊断临界值时，红细胞凝集素免疫试验（Erythro-LIT）对脑脊液的敏感性和特异性分别为92%和100%。4. 此处展示的高敏感性和特异性，连同该试验的简单性和低成本，使红细胞凝集素免疫试验（Erythro-LIT）成为筛查神经囊尾蚴病特异性抗体的一种潜在有用方法。