揭示油棕转运RNA修饰及其在果实成熟过程中的动态变化。

Unveiling transfer RNA modifications of oil palm and their dynamic changes during fruit ripening.

作者信息

Deng Dehai, Qin Yichao, Lin Xiuying, Chu Meng, Lv Daizhu, Lin Huan

机构信息

State Key Laboratory of Marine Resource Utilization in South China Sea, Hainan University, Haikou, 570228, P. R. China.

School of Ecology and Environment, Hainan University, Haikou, P. R. China.

出版信息

BMC Plant Biol. 2025 Mar 29;25(1):398. doi: 10.1186/s12870-025-06426-9.

DOI:10.1186/s12870-025-06426-9

PMID:40155815

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11954249/

Abstract

BACKGROUND

The oil palm (Elaeis guineensis) is a crucial agricultural commodity, yielding the highest oil output among oil-bearing crops. Despite its significance, productivity challenges persist due to genetic and environmental factors. This study breaks new ground by mapping tRNA modifications in oil palm, exploring their roles during fruit ripening, an area not extensively studied in non-model crops.

RESULTS

Utilizing advanced RNA mass spectrometry techniques, we identified 48 distinct tRNA modifications across 88 sites, alongside 164 genes associated with tRNA modifying enzymes. This comprehensive mapping reveals the decreasing nature of most tRNA modifications during fruit development, except for adenosine 2'-O methylation (Am). It hints at a gradual weakening of protein translation quality control and highlights a unique role for Am during fruit ripening. Additionally, lipidomic analysis tracked 674 lipids in oil palm fruits, indicating a correlation between tRNA modifications and the accumulation of specific lipids.

CONCLUSIONS

This study mapped tRNA modifications in oil palm for the first time and showed the diversity of dynamic changes in tRNA modifications as the fruits develop.

摘要

背景

油棕（Elaeis guineensis）是一种重要的农产品，在含油作物中产量最高。尽管其具有重要意义，但由于遗传和环境因素，生产力挑战依然存在。本研究通过绘制油棕中的tRNA修饰图谱，探索它们在果实成熟过程中的作用，在非模式作物中尚未广泛研究的这一领域开辟了新的道路。

结果

利用先进的RNA质谱技术，我们在88个位点鉴定出48种不同的tRNA修饰，以及164个与tRNA修饰酶相关的基因。这一全面的图谱揭示了在果实发育过程中，除了腺苷2'-O甲基化（Am）外，大多数tRNA修饰呈下降趋势。这暗示了蛋白质翻译质量控制的逐渐减弱，并突出了Am在果实成熟过程中的独特作用。此外，脂质组学分析追踪了油棕果实中的674种脂质，表明tRNA修饰与特定脂质的积累之间存在相关性。