Cosenza Gianfranco, Fulgione Andrea, Albarella Sara, Ciotola Francesca, Peretti Vincenzo, Gallo Daniela, Pauciullo Alfredo
Department of Agricultural Science, University of Naples Federico II, Piazza Carlo Di Borbone 1, Portici, 80055, Italy.
Department of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 1, Naples, 80137, Italy.
BMC Vet Res. 2025 Mar 28;21(1):215. doi: 10.1186/s12917-025-04694-z.
Over the past thirty years, the identification of species-specific molecular markers has significantly advanced our understanding of genetic diversity in both plants and animals. Among these, short InDels have emerged as vital genomic features, contributing more to sequence divergence than single nucleotide polymorphisms do in closely related species. This study aimed to identify specific InDels for Bos taurus, Bubalus bubalis, Capra hircus, and Ovis aries via an in silico approach and validated them in 400 individuals (100 for each species).
We identified and characterized short, specific InDels in the sequences of the CSN1S1, CSN1S2, MSTN, and PRLR genes, which can be used for species identification of Capra hircus, Ovis aries, Bos taurus, and Bubalus bubalis, respectively. We developed a Tetraplex Specific PCR assay to enable efficient discrimination among these species.
This study highlights the utility of InDels as biallelic, codominant markers that are cost-effective and easy to analyse, providing valuable tools for genetic diversity analysis and species identification.
在过去三十年中，物种特异性分子标记的鉴定极大地推动了我们对植物和动物遗传多样性的理解。其中，短插入缺失已成为重要的基因组特征，在亲缘关系较近的物种中，其对序列差异的贡献比单核苷酸多态性更大。本研究旨在通过计算机模拟方法鉴定牛、水牛、山羊和绵羊的特定插入缺失，并在400个个体（每个物种100个）中进行验证。
我们在CSN1S1、CSN1S2、MSTN和PRLR基因序列中鉴定并表征了短的、特定的插入缺失，这些插入缺失可分别用于山羊、绵羊、牛和水牛的物种鉴定。我们开发了一种四重特异性PCR检测方法，以实现对这些物种的有效区分。
本研究强调了插入缺失作为双等位基因、共显性标记的实用性，这些标记具有成本效益且易于分析，为遗传多样性分析和物种鉴定提供了有价值的工具。
