Tang Yan, Wang Hai-Tao, Li Jing-Hao, Hou Qing-Yu, Qin Si-Yuan, Ma He, Qin Ya, Zhao Quan, Elsheikha Hany M, Liu Shuo
College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou Province, PR China.
College of Life Sciences, Changchun Sci-Tech University, Shuangyang, Jilin Province, PR China.
BMC Vet Res. 2025 Mar 28;21(1):218. doi: 10.1186/s12917-025-04604-3.
Pentatrichomonas hominis is a zoonotic pathogen linked to gastrointestinal diseases in various animal species and humans. However, its prevalence of in wild rodents remains unexplored. Therefore, investigating the prevalence and molecular characteristics of P. hominis in wild rodents is essential.
M. Replace the crossed out sentence above with the following version:This study assessed the prevalence of P. hominis in wild rodents by analyzing 510 fecal samples using nested PCR. Statistical analysis of risk factors was performed with SAS (v9.0) and SPSS software.
The infection rates were 18.18% (16/88) in Yunnan, 0.94% (3/319) in Hunan, and 0% (0/103) in Guangxi. Among the species, Rattus rattus sladeni had the highest infection rate at 40% (2/5), followed by R. flavipectus at 23.08% (9/39), while Niviventer lotipes (0%, 0/23) and R. losea (0%, 0/41) showed no infection. Seasonally, the highest prevalence was observed in autumn (18.18%, 16/88) and the lowest in winter (0%, 0/103). Rodents from farmland had significantly higher infection rates than mountain areas (0%, 0/103) and lakeshores (0.32%, 1/312). Although female rodents had a higher infection rate (4.62%, 11/238) compared to males (2.94%, 8/272), the difference was not statistically significant. Phylogenetic analysis revealed that all P. hominis strains identified belonged to the zoonotic CC1 genotype.
This study is the first to describe the distribution of P. hominis in wild rodents, paving the way for further epidemiological research on this parasite in wild animals. Such research is crucial for developing strategies to protect human health from zoonotic threats from wild rodents.
人五毛滴虫是一种人畜共患病原体，与多种动物和人类的胃肠道疾病有关。然而，其在野生啮齿动物中的流行情况仍未得到探索。因此，调查野生啮齿动物中人五毛滴虫的流行情况和分子特征至关重要。
本研究通过巢式PCR分析510份粪便样本，评估野生啮齿动物中人五毛滴虫的流行情况。使用SAS（v9.0）和SPSS软件对危险因素进行统计分析。
云南的感染率为18.18%（16/88），湖南为0.94%（3/319），广西为0%（0/103）。在这些物种中，斯氏家鼠的感染率最高，为40%（2/5），其次是黄胸鼠，为23.08%（9/39），而社鼠（0%，0/23）和黄毛鼠（0%，0/41）未显示感染。按季节来看，秋季的流行率最高（18.18%，16/88），冬季最低（0%，0/103）。农田中的啮齿动物感染率明显高于山区（0%，0/103）和湖滨（0.32%，1/312）。尽管雌性啮齿动物的感染率（4.62%，11/238）高于雄性（2.94%，8/272），但差异无统计学意义。系统发育分析表明，所有鉴定出的人五毛滴虫菌株均属于人畜共患的CC1基因型。
本研究首次描述了人五毛滴虫在野生啮齿动物中的分布，为进一步开展该寄生虫在野生动物中的流行病学研究铺平了道路。此类研究对于制定保护人类健康免受野生啮齿动物人畜共患威胁的策略至关重要。
