肝胆疾病患者血清中3β-羟基-5-胆烯酸及其葡萄糖醛酸化和硫酸化结合物的测定

Determination of 3 beta-hydroxy-5-cholenoic acid in serum of hepatobiliary diseases--its glucuronidated and sulfated conjugates.

作者信息

Takikawa H, Otsuka H, Beppu T, Seyama Y

出版信息

Biochem Med. 1985 Jun;33(3):393-400. doi: 10.1016/0006-2944(85)90016-x.

Abstract

3 beta-Hydroxy-5-cholenoic acid in the serum of control subjects and 62 patients with various hepatobiliary diseases was quantitated by mass fragmentography after separation into nonglucuronidated-nonsulfated, glucuronidated, and sulfated fractions. Deuterium-labeled deoxycholic acid and its glucuronide and sulfate were used as internal standards. Mean concentrations of total 3 beta-hydroxy-5-cholenoic acid in serum (mumole/liter) were as follows: Control subjects (14), 0.184; obstructive jaundice (15), 6.783; liver cirrhosis, compensated (12), 0.433, and decompensated (12), 1.636; chronic hepatitis (12), 0.241; and acute hepatitis (11), 2.364. Most of the 3 beta-hydroxy-5-cholenoic acid was glucuronidated or sulfated. Only in patients with obstructive jaundice did glucuronidation (60 +/- 14%) exceed sulfation (31 +/- 14%), sulfation exceeding glucuronidation in the others. The UDP-glucuronyltransferase might have different substrate specificities for 3 beta-hydroxy-5-cholenoic acid and other common bile acids, especially in the cholestatic state.

摘要

将对照受试者及62例患有各种肝胆疾病患者血清中的3β-羟基-5-胆烯酸分离为非葡萄糖醛酸化-非硫酸化、葡萄糖醛酸化和硫酸化组分后，采用质量碎片谱法进行定量分析。以氘标记的脱氧胆酸及其葡萄糖醛酸苷和硫酸盐作为内标。血清中总3β-羟基-5-胆烯酸的平均浓度（微摩尔/升）如下：对照受试者（14例），0.184；梗阻性黄疸患者（15例），6.783；代偿期肝硬化患者（12例），0.433，失代偿期肝硬化患者（12例），1.636；慢性肝炎患者（12例），0.241；急性肝炎患者（11例），2.364。大部分3β-羟基-5-胆烯酸被葡萄糖醛酸化或硫酸化。仅在梗阻性黄疸患者中，葡萄糖醛酸化（60±14%）超过硫酸化（31±14%），在其他患者中硫酸化超过葡萄糖醛酸化。UDP-葡萄糖醛酸基转移酶对3β-羟基-5-胆烯酸和其他常见胆汁酸可能具有不同的底物特异性，尤其是在胆汁淤积状态下。