Pike S F, Shephard E A, Rabin B R, Phillips I R
Biochem Pharmacol. 1985 Jul 15;34(14):2489-94. doi: 10.1016/0006-2952(85)90531-3.
We have previously shown that the 43-fold induction by phenobarbital of the major phenobarbital-inducible cytochrome P-450 of rat liver microsomal membranes (PB P-450) is mediated by a 20-fold increase in the amount of its mRNA in the cytoplasm. Here we demonstrate that the induction of the mRNA can be almost entirely accounted for by an increase in the rate of transcription of genes coding for PB P-450, and involves little or no change in the rates of processing, transport or degradation of the mRNA. Phenobarbital treatment resulted in no amplification or rearrangement of PB P-450 genes.
我们之前已经表明，苯巴比妥对大鼠肝微粒体膜主要的苯巴比妥诱导型细胞色素P-450（PB P-450）的43倍诱导作用，是由其细胞质中mRNA量增加20倍介导的。在此我们证明，mRNA的诱导几乎完全可归因于编码PB P-450的基因转录速率的增加，并且在mRNA的加工、转运或降解速率方面几乎没有变化。苯巴比妥处理并未导致PB P-450基因的扩增或重排。
