Kanwal Nazish, Khan Mansoor, Khan Saeed Ahmad, Bari Ahmed, Ali Essam A, Sun Wei, Rehman Tanzila, Nishan Umar, Badshah Amir
Department of Chemistry, Kohat University of Science and Technology, Kohat, 26000, KP Pakistan.
Department of Pharmacy, University of Science and Technology, Kohat, 26000, KP Pakistan.
Anal Biochem. 2025 Jul;702:115855. doi: 10.1016/j.ab.2025.115855. Epub 2025 Mar 27.
Ascorbic acid is implicated in various diseases such as scurvy, oxidative stress, cardiovascular diseases, etc. Herein, for the first time, a simple and efficient strategy was used to synthesize cross-linked chitosan-stabilized copper oxide nanoparticles (CuO@C-CS) as a non-toxic and biodegradable-based approach. Various spectroscopic techniques, including FTIR, XRD, SEM, EDX, TGA, and elemental mapping, confirmed the synthesis of the material. The synthesized nanozyme (CuO@C-CS) was used as a peroxidase mimic for the detection of ascorbic acid, through the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB) with the assistance of hydrogen peroxide. The synthesized mimic enzyme transforms colorless TMB into oxTMB. The sensing of ascorbic acid was achieved through the peroxidase-like inhibitory activity of the mimic enzyme along with the reduction of oxTMB. The sensor system was fine-tuned, and it showed a limit of detection, a limit of quantification, a linear range, and regression coefficient values of 0.24 μM, 0.80 μM, 1-96 μM, and 0.999, respectively. The fabricated sensor was very selective in the presence of various potential interferents. The proposed sensor was successfully applied to commercially available orange juices for the qualitative and quantitative determination of ascorbic acid. The sensor can be used for the determination of ascorbic acid in biomedical and food samples.
抗坏血酸与多种疾病有关，如坏血病、氧化应激、心血管疾病等。在此，首次采用一种简单有效的策略合成了交联壳聚糖稳定的氧化铜纳米颗粒（CuO@C-CS），这是一种基于无毒且可生物降解的方法。包括傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）、扫描电子显微镜（SEM）、能量散射X射线光谱（EDX）、热重分析（TGA）和元素映射在内的各种光谱技术证实了该材料的合成。合成的纳米酶（CuO@C-CS）在过氧化氢的辅助下，通过显色底物3,3',5,5'-四甲基联苯胺（TMB）用作抗坏血酸检测的过氧化物酶模拟物。合成的模拟酶将无色的TMB转化为氧化型TMB（oxTMB）。通过模拟酶的过氧化物酶样抑制活性以及oxTMB的还原实现了抗坏血酸的传感。对传感器系统进行了优化调整，其检测限、定量限、线性范围和回归系数值分别为0.24 μM、0.80 μM、1 - 96 μM和0.999。所制备的传感器在存在各种潜在干扰物的情况下具有很高的选择性。所提出的传感器已成功应用于市售橙汁中抗坏血酸的定性和定量测定。该传感器可用于生物医学和食品样品中抗坏血酸的测定。
