Santana-Oliveira Daiana Araujo, Souza-Tavares Henrique, Fernandes-da-Silva Aline, Marinho Thatiany Souza, Silva-Veiga Flavia Maria, Daleprane Julio Beltrame, Souza-Mello Vanessa
Laboratory of Morphometry, Metabolism and Cardiovascular Diseases, Biomedical Center, Institute of Biology. Rio de Janeiro State University, Rio de Janeiro, Brazil.
Laboratory for Studies of Interactions Between Nutrition and Genetics (LEING), Institute of Nutrition, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Mol Cell Endocrinol. 2025 May 15;602:112533. doi: 10.1016/j.mce.2025.112533. Epub 2025 Mar 27.
This study evaluated the effects of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on UCP1-dependent and UCP1-independent thermogenic and mitochondrial dynamics markers in the inguinal sWAT of high-fat-fed mice.
Sixty male C57BL/6 mice (3 months old) were divided into six experimental groups: control diet (C), C + HIIT (C-HIIT), C + MICT (C-MICT), high-fat diet (HF), HF + HIIT (HF-HIIT) and HF + MICT (HF-MICT). The diet and exercise protocols started simultaneously and lasted ten weeks.
HIIT and MICT prevented body mass gain and fat pad expansion, improved insulin sensitivity, and induced browning in C-fed and HF-fed animals. Chronic intake of a HF diet caused adipocyte hypertrophy with a proinflammatory adipokine profile and impaired the expression of thermogenic and mitochondrial dynamics markers. However, both exercise intensities increased anti-inflammatory adipokine concentrations and improved gene markers of mitochondrial dynamics, resulting in sustained UCP1-dependent and UCP1-independent thermogenic markers and maintenance of the beige phenotype in inguinal sWAT. The principal component analysis placed all trained groups opposite the HF group and near the C group, ensuring the effectiveness of HIIT and MICT to prevent metabolic alterations.
This study provides reliable evidence that, regardless of intensity, exercise is a strategy to prevent obesity by reducing body fat accumulation and inducing browning. The anti-inflammatory adipokine profile and the increased expression of UCP1-dependent and UCP1-independent thermogenic markers sustained active beige adipocytes and mitochondrial enhancement to halt metabolic disturbances due to HF-feeding in exercised mice.
本研究评估了高强度间歇训练（HIIT）和中等强度持续训练（MICT）对高脂喂养小鼠腹股沟皮下白色脂肪组织（sWAT）中依赖解偶联蛋白1（UCP1）和不依赖UCP1的产热及线粒体动力学标志物的影响。
将60只3月龄雄性C57BL/6小鼠分为6个实验组：对照饮食组（C）、C + HIIT组（C - HIIT）、C + MICT组（C - MICT）、高脂饮食组（HF）、HF + HIIT组（HF - HIIT）和HF + MICT组（HF - MICT）。饮食和运动方案同时开始，持续10周。
HIIT和MICT可防止C喂养和HF喂养动物体重增加和脂肪垫扩大，改善胰岛素敏感性，并诱导褐色化。长期摄入高脂饮食会导致脂肪细胞肥大，伴有促炎脂肪因子谱，并损害产热和线粒体动力学标志物的表达。然而，两种运动强度均增加了抗炎脂肪因子浓度，改善了线粒体动力学的基因标志物，导致腹股沟sWAT中依赖UCP1和不依赖UCP1的产热标志物持续存在，并维持米色表型。主成分分析将所有训练组置于HF组对面且靠近C组，确保了HIIT和MICT预防代谢改变的有效性。
本研究提供了可靠证据，即无论强度如何，运动都是通过减少体脂积累和诱导褐色化来预防肥胖的一种策略。抗炎脂肪因子谱以及依赖UCP1和不依赖UCP1的产热标志物表达增加，维持了活跃的米色脂肪细胞和线粒体增强，以阻止运动小鼠因高脂喂养引起的代谢紊乱。
