Dewey S L, West J R
Alcohol. 1985 Mar-Apr;2(2):221-5. doi: 10.1016/0741-8329(85)90049-7.
The entorhinal (perforant path) projection to the dentate gyrus was labeled with an anterograde horseradish peroxidase method to test whether prenatal exposure to ethanol affected the normal development of afferent lamination. Mean ethanol consumption of the ethanol-consuming dams was 12.7 g/kg +/- 0.3 g per day during days 1-21 of gestation. Adult offspring of normal and pair-fed controls as well as ethanol-exposed rats were analyzed. Computer-assisted image analysis of the entorhinal terminal field organization revealed no permanent changes in the development of the afferent lamination pattern in the dentate gyrus molecular layer in spite of the heavy in utero ethanol exposure.
采用顺行辣根过氧化物酶法标记内嗅皮质（穿通通路）至齿状回的投射，以检测产前暴露于乙醇是否会影响传入纤维分层的正常发育。在妊娠第1至21天，摄入乙醇的母鼠平均乙醇摄入量为每天12.7 g/kg±0.3 g。对正常对照组、配对喂养对照组以及暴露于乙醇的大鼠的成年后代进行了分析。尽管子宫内大量暴露于乙醇，但通过计算机辅助图像分析内嗅皮质终末野的组织情况发现，齿状回分子层传入纤维分层模式的发育没有永久性变化。
