Dewey S L, West J R
Alcohol. 1985 Jul-Aug;2(4):617-22. doi: 10.1016/0741-8329(85)90089-8.
The anterograde horseradish peroxidase method was used to determine if prenatal exposure to ethanol affected the development of the characteristic afferent lamination pattern of the commissural projection to the dentate gyrus. Mean ethanol consumption for the ethanol-consuming dams was 12.7 g/kg +/- 0.3 g per day. Adult offspring of rats that consumed a liquid diet containing 35% ethanol-derived calories during days 1-21 of gestation, and both pair-fed and normal controls were examined. Brain weights and volumes of the ethanol and pair-fed control rats did not differ significantly from normal controls. However, body weights of ethanol-exposed rats were significantly reduced compared to normal controls. Computer-assisted image analysis of the HRP-labeling revealed that in spite of the heavy ethanol exposure there was no evidence of alterations in the spatial distribution of the commissural terminal field.
采用顺行性辣根过氧化物酶法来确定孕期暴露于乙醇是否会影响连合纤维投射至齿状回的特征性传入纤维分层模式的发育。摄入乙醇的母鼠平均乙醇摄入量为每天12.7克/千克±0.3克。对在妊娠第1至21天食用含35%乙醇热量液体饲料的大鼠的成年后代，以及配对喂养的对照组和正常对照组进行了检查。乙醇组和配对喂养对照组大鼠的脑重量和体积与正常对照组相比无显著差异。然而，与正常对照组相比，暴露于乙醇的大鼠体重显著降低。对辣根过氧化物酶标记进行计算机辅助图像分析显示，尽管乙醇暴露量很大，但没有证据表明连合终末场的空间分布发生改变。
