Mansouri Fatemeh, Feliziani Giulia, Bordoni Laura, Gabbianelli Rosita
School of Advanced Studies, University of Camerino, Camerino, Italy; Unit of Molecular Biology and Nutrigenomics, School of Pharmacy, University of Camerino, Camerino, Italy.
Unit of Molecular Biology and Nutrigenomics, School of Pharmacy, University of Camerino, Camerino, Italy.
J Acad Nutr Diet. 2025 Mar 28. doi: 10.1016/j.jand.2025.03.011.
Resveratrol, a natural polyphenol compound, possesses anti-aging, antitumor, and vascular protective properties. These attributes are believed to stem from its influence on Sirtuin 1 (Sirt1), a member of the human Sirtuin family and a nicotinamide adenine dinucleotide-dependent histone deacetylase.
The aim of this study was to quantitatively investigate the impact of resveratrol supplementation on Sirt1 levels in adults by conducting a systematic review and meta-analysis of randomized controlled trials (RCTs) involving resveratrol supplementation.
This Grading of Recommendations Assessment, Development and Evaluation-assessed systematic review involved a comprehensive search of PubMed, Embase, MEDLINE, Scopus, Web of Science, Cochrane Central Register of Controlled Trials, and Google Scholar databases using related keywords and was conducted from March 14, 2024, to April 15, 2024, to identify all RCTs investigating resveratrol's effects on Sirt1. Effect sizes were quantified as mean differences (MDs) or standardized mean differences (SMDs), with standard deviations of outcomes. An overall effect estimate was derived using a random-effects model when 2 or more studies reported similar outcomes. Statistical heterogeneity was assessed through the calculation of I statistics. In addition, a dose-response analysis was performed to assess potential dose-response relationships. Risk of bias was assessed using the Cochrane risk-of-bias tool for RCTs (RoB 2). Publication bias was evaluated using Begg's test and a meta-regression using the year of publication as a moderator.
Eleven RCTs examining the effects of resveratrol on Sirt1 gene expression (4 RCTs), protein expression (5 RCTs), and serum levels (3 RCTs) were included in the meta-analysis. The results showed no significant impact of resveratrol on Sirt1 gene expression (SMD = 0.05; 95% CI -0.24 to 0.344; P = .73), protein expression (SMD = 0.3; 95% CI -0.15 to 0.77; P = .18), or serum levels (MD = -0.04; 95% CI -0.235 to 0.16; P = .7). However, subgroup analyses suggested a significant increase in Sirt1 gene expression in studies with an intervention duration of <12 weeks and evaluating blood tissue. Furthermore, the impact of resveratrol on Sirt1 appeared to be influenced by the dosage regimen, with a significant effect for intervention duration.
Study results indicate that resveratrol supplementation does not significantly influence human Sirt1 based on the overall meta-analysis. However, the dose-response analysis suggests that the effect of resveratrol on Sirt1 depends on the dosage regimen.
白藜芦醇是一种天然多酚化合物，具有抗衰老、抗肿瘤和血管保护特性。这些特性被认为源于其对沉默调节蛋白1（Sirt1）的影响，Sirt1是人类沉默调节蛋白家族的成员，也是一种烟酰胺腺嘌呤二核苷酸依赖性组蛋白脱乙酰酶。
本研究的目的是通过对涉及补充白藜芦醇的随机对照试验（RCT）进行系统评价和荟萃分析，定量研究补充白藜芦醇对成年人Sirt1水平的影响。
本项基于推荐分级评估、制定与评价的系统评价，使用相关关键词全面检索了PubMed、Embase、MEDLINE、Scopus、科学网、Cochrane对照试验中央注册库和谷歌学术数据库，检索时间为2024年3月14日至2024年4月15日，以识别所有研究白藜芦醇对Sirt1影响的RCT。效应大小定量为平均差（MDs）或标准化平均差（SMDs），并给出结果的标准差。当两项或更多研究报告相似结果时，使用随机效应模型得出总体效应估计值。通过计算I统计量评估统计异质性。此外，进行剂量反应分析以评估潜在的剂量反应关系。使用Cochrane随机对照试验偏倚风险工具（RoB 2）评估偏倚风险。使用Begg检验和以发表年份为调节变量的荟萃回归评估发表偏倚。
荟萃分析纳入了11项研究白藜芦醇对Sirt1基因表达（4项RCT）、蛋白表达（5项RCT）和血清水平（3项RCT）影响的RCT。结果显示，白藜芦醇对Sirt1基因表达（SMD = 0.05；95%CI -0.24至0.344；P = 0.73）、蛋白表达（SMD = 0.3；95%CI -0.15至0.77；P = 0.18）或血清水平（MD = -0.04；95%CI -0.235至0.16；P = 0.7）无显著影响。然而，亚组分析表明，在干预持续时间<12周且评估血液组织的研究中，Sirt1基因表达显著增加。此外，白藜芦醇对Sirt1的影响似乎受给药方案影响，干预持续时间有显著影响。
研究结果表明，基于总体荟萃分析，补充白藜芦醇对人类Sirt1无显著影响。然而，剂量反应分析表明，白藜芦醇对Sirt1的影响取决于给药方案。
