Szulc Justyna, Grzyb Tomasz, Gutarowska Beata, Nizioł Joanna, Krupa Sumi, Ruman Tomasz
Department of Environmental Biotechnology, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Wólczańska Street 171/173, 90-530 Łódź, Poland.
Department of Inorganic and Analytical Chemistry, Faculty of Chemistry, Rzeszów University of Technology, Powstańców Warszawy Street 6, 35-959 Rzeszów, Poland.
J Agric Food Chem. 2025 Apr 9;73(14):8225-8242. doi: 10.1021/acs.jafc.5c00349. Epub 2025 Mar 30.
The aim of this study was to evaluate innovative mass spectrometry imaging (MSI) for determining the metabolic potential of selected soil bacteria from the genera and in the presence of the phytopathogen . This research marks the first application of direct 3D MSI that to visualize interactions between potential biocontrol agents and plant pathogens on agar medium. The LARAPPI/CI-3D-MSI (Laser-Assisted Remote Atmospheric Pressure Imaging/Chemical Ionization-3D Mass Spectrometry Imaging) setup provided valuable insights into the compounds produced by the tested microorganisms, revealing their spatial distributions and their ability to diffuse into the agar medium. Subsequently, a Pathway Impact Analysis of Metabolites was conducted. Ion images based on ultrahigh resolution mass spectrometry data were obtained, including for potentially bioactive compounds. Statistical analysis of the entire MS data set showed that the metabolites identified for samples were distinctly separated from the samples and could be helpful tools for screening biocontrol strains. The LARAPPI/CI MSI technique offers significant advantages over existing MSI methods. Further research using this technology could help validate the effectiveness of various biopreparations and contribute to enhancing the quality of biological plant protection products available on the market.
本研究的目的是评估创新的质谱成像（MSI）技术，以确定在植物病原体存在的情况下，从特定属中筛选出的土壤细菌的代谢潜力。本研究标志着直接三维质谱成像首次应用于可视化琼脂培养基上潜在生物防治剂与植物病原体之间的相互作用。LARAPPI/CI-3D-MSI（激光辅助远程大气压成像/化学电离三维质谱成像）装置为测试微生物产生的化合物提供了有价值的见解，揭示了它们的空间分布以及扩散到琼脂培养基中的能力。随后，进行了代谢物的途径影响分析。基于超高分辨率质谱数据获得了离子图像，包括潜在生物活性化合物的离子图像。对整个质谱数据集的统计分析表明，为样本鉴定的代谢物与样本明显分离，可能成为筛选生物防治菌株的有用工具。与现有的质谱成像方法相比，LARAPPI/CI MSI技术具有显著优势。使用该技术的进一步研究有助于验证各种生物制剂的有效性，并有助于提高市场上现有生物植物保护产品的质量。
