Perek Zofia, Krupa Sumi, Nizioł Joanna, Kręgiel Dorota, Ruman Tomasz, Gutarowska Beata
Department of Environmental Biotechnology, Lodz University of Technology, Wolczanska 171/173, 90-530 Lodz, Poland.
Interdisciplinary Doctoral School, Lodz University of Technology, Zeromskiego 116, 90-924 Lodz, Poland.
Molecules. 2025 Aug 4;30(15):3268. doi: 10.3390/molecules30153268.
One of the most important features of is its strong antimicrobial activity against phytopathogens, which makes it a suitable candidate for use in biocontrol during crop cultivation. However, the mechanisms of its antimicrobial activity are not currently well understood. In this study, we used metabolomic methods to investigate the possible mechanisms of antimicrobial activity by against phytopathogenic fungi. First, we tested the antimicrobial activity of five selected isolates against eleven phytopathogenic molds. Based on the results, selected yeast-pathogen co-cultures were cultivated on liquid and solid media. The supernatants from the liquid co-cultures were analyzed using the UHPLC-QToF-UHRMS and MS/MS methods. Co-culture growth on solid agar media was examined using the LARAPPI/CI MSI method. The yeast exhibited strong antagonism toward the mold phytopathogens. The LARAPPI/CI MSI method revealed the presence of various compounds with potential antifungal activity. The complex UHPLC-QToF-UHRMS analysis confirmed that the metabolic response of depends on specific yeast-pathogen interactions.
[具体物质名称]最重要的特征之一是其对植物病原体具有强大的抗菌活性，这使其成为作物种植过程中生物防治的合适候选物。然而，目前其抗菌活性的机制尚不清楚。在本研究中，我们使用代谢组学方法来研究[具体物质名称]对植物病原真菌抗菌活性的可能机制。首先，我们测试了五个选定菌株对十一种植物病原霉菌的抗菌活性。根据结果，在液体和固体培养基上培养选定的酵母 - 病原体共培养物。使用UHPLC - QToF - UHRMS和MS/MS方法分析液体共培养物的上清液。使用LARAPPI/CI MSI方法检查在固体琼脂培养基上的共培养生长情况。该酵母对霉菌植物病原体表现出强烈的拮抗作用。LARAPPI/CI MSI方法揭示了存在具有潜在抗真菌活性的各种化合物。复杂的UHPLC - QToF - UHRMS分析证实，[具体物质名称]的代谢反应取决于特定的酵母 - 病原体相互作用。
