Schmudlach Andrew, Spear Saralynn, Hua Yimin, Fertier-Prizzon Stephanie, Kochling Jianmei
Analytical Development, mRNA Center of Excellence, Sanofi, Waltham, United States.
Analytical Sciences, mRNA Center of Excellence, Sanofi, Marcy l'Etoile, France.
Biol Methods Protoc. 2025 Mar 20;10(1):bpaf021. doi: 10.1093/biomethods/bpaf021. eCollection 2025.
Oligonucleotide integrity is a critical quality attribute for many new therapeutic modalities. Current assays often measure attributes such as length using capillary electrophoresis or liquid chromatography. The length is then corroborated with sequencing data to ensure oligonucleotide quality. An orthogonal measure to these classical separations is to measure intact mass, which traditional mass spectrometry cannot. Herein, we report the use of mass photometry to directly measure RNA length using RNA ladders as a calibrant.
寡核苷酸完整性是许多新治疗方式的关键质量属性。当前的检测方法通常使用毛细管电泳或液相色谱来测量诸如长度等属性。然后将长度与测序数据进行比对，以确保寡核苷酸质量。与这些传统分离方法不同的一种测量方法是测量完整质量，而传统质谱无法做到这一点。在此，我们报告了使用质量光度法以RNA梯作为校准物直接测量RNA长度。
