Hummel B C, Walfish P G
Biochim Biophys Acta. 1985 Aug 16;841(2):173-85. doi: 10.1016/0304-4165(85)90019-4.
Iodothyronine deiodinase activities of rat liver microsomes prepared by (1) differential centrifugation, (2) column chromatography, (3) precipitation with Ca2+, (4) precipitation at low pH, or combinations of these were compared. Method 2 or 2 followed by 4 provided microsomes with specific activities 4.6- and 7.4-times higher than method 1, respectively. Both Triton X-100 at 0.1% (w/v) and 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (Chaps) at 4-6 mM efficiently solubilized deiodinase and were not inhibitory at low concentrations. The Chaps-soluble enzyme could be moderately purified by fractionation with ammonium sulfate but more effectively with poly(ethylene glycol).
(1) 差速离心法，(2) 柱色谱法，(3) 用Ca2+沉淀法，(4) 在低pH下沉淀法，或这些方法的组合。方法2或方法2之后接着方法4所得到的微粒体的比活性分别比方法1高4.6倍和7.4倍。0.1%（w/v）的 Triton X-100和4-6 mM的3-[(3-胆酰胺丙基)二甲基铵]-1-丙烷磺酸盐（Chaps）都能有效地溶解脱碘酶，并且在低浓度下没有抑制作用。Chaps可溶酶可以通过硫酸铵分级分离进行适度纯化，但用聚乙二醇进行纯化更有效。
