Pfister Sonia, Walter Sophie, Perrier Aurélie, Collot Mayeul
Chemistry of Photoresponsive Systems, Laboratoire de Chémo-Biologie Synthétique et Thérapeutique (CBST) UMR 7199, CNRS, Université de Strasbourg, F-67400 Illkirch, France.
Laboratoire Interdisciplinaire des Energies de Demain (LIED), UMR 8236, CNRS, Université Paris Cité, F-75013, Paris, France.
Chem Commun (Camb). 2025 Apr 17;61(33):6170-6173. doi: 10.1039/d5cc00151j.
We have developed spontaneously blinking fluorescent probes based on the reversible spirolactamization of rhodamine, to efficiently image the plasma membrane (PM) of live cells with enhanced resolution using SMLM. This study demonstrates that the blinking efficiency of spiroamide PM probes is not solely governed by their p; the presence of a charged polar group on the amide should also be taken into account.
我们基于罗丹明的可逆螺内酰胺化反应开发了自发闪烁的荧光探针，以便使用超分辨显微镜（SMLM）以更高的分辨率对活细胞的质膜（PM）进行高效成像。本研究表明，螺酰胺质膜探针的闪烁效率不仅取决于其p值；酰胺上带电极性基团的存在也应予以考虑。
