Mora Alejandro, García-Bernal David, Rodríguez-Lozano Francisco Javier, Ghilotti James, Lozano Adrián, López-García Sergio
Department of Dermatology, Stomatology, Radiology and Physical Medicine, Morales Meseguer Hospital, Faculty of Medicine, University of Murcia, Murcia 30008, Spain.
Department of Biochemistry, Molecular Biology B and Immunology, Faculty of Medicine, University of Murcia, Biomedical Research Institute (IMIB), Murcia 30120, Spain.
Dent Mater. 2025 Jun;41(6):644-657. doi: 10.1016/j.dental.2025.03.011. Epub 2025 Mar 30.
To evaluate the cytocompatibility, bioactivity, and mineralization potential of new tricalcium silicate-based Material (Biodentine XP) on human dental pulp stem cells (hDPSCs) compared to other calcium silicate-based materials (MTA-Ang and Ther-PT).
Standardized discs and 1:1, 1:2, and 1:4 eluates of Biodentine XP (BD-XP), MTA-Ang, and Ther-PT were prepared after setting. Human dental pulp stem cells (hDPSCs) were isolated from extracted third molars of healthy patients and cultured under standard conditions. The following assays were performed: cell attachment and morphology were assessed by scanning electron microscopy (SEM); metabolic activity and cell viability was evaluated by MTT and cell cycle analysis; cellular calcium ion content was analyzed by energy dispersive X-ray (EDX) and calcium release analysis; cell migration/proliferation by wound healing assays; cytoskeletal organization, analysis of cell apoptosis and necrosis, reactive oxygen species production, osteogenic marker expression were quantified by flow cytometry and RT-qPCR; and cell mineralization potential was determined through Alizarin Red S staining. Comparisons were made with hDPSCs cultured in unconditioned media (negative control) and osteogenic culture media (positive control). Statistical significance was set at p < 0.05.
Biodentine XP (BD-XP) showed significantly positive results in cytocompatibility assays, including cell metabolic activity, viability, attachment, and morphology, compared to the negative control group, whereas MTA-Ang and Ther-PT showed moderate results. BD-XP exhibited a significant upregulation of osteogenic markers, including ALP and DSPP, compared to both the negative and positive control groups. In terms of mineralization potential, BD-XP-treated cells showed significantly higher calcified nodule formation compared to MTA-Ang, Ther-PT, the negative control, and the positive control groups.
BD-XP exhibits superior cytocompatibility and promotes osteo/odontogenic differentiation of human dental pulp stem cells (hDPSCs). BD-XP significantly enhances the upregulation of osteo/odontogenic markers such as ALP and DSPP, and promotes calcified nodule formation more effectively than MTA-Ang and Ther-PT. The high calcium ion content of BD-XP plays a key role in its ability to promote cellular adhesion, proliferation, and mineralization, making it a highly effective material for regenerative dental applications. These findings provide strong evidence to support the use of Biodentine XP in regenerative dental therapies, particularly in vital pulp treatments, where enhanced cellular adhesion and mineralization are critical for clinical success.
与其他硅酸钙基材料（MTA-Ang和Ther-PT）相比，评估新型硅酸三钙基材料（Biodentine XP）对人牙髓干细胞（hDPSCs）的细胞相容性、生物活性和矿化潜力。
凝固后制备Biodentine XP（BD-XP）、MTA-Ang和Ther-PT的标准化圆盘以及1:1、1:2和1:4洗脱液。从健康患者拔除的第三磨牙中分离出人牙髓干细胞（hDPSCs），并在标准条件下培养。进行了以下检测：通过扫描电子显微镜（SEM）评估细胞附着和形态；通过MTT和细胞周期分析评估代谢活性和细胞活力；通过能量色散X射线（EDX）和钙释放分析分析细胞钙离子含量；通过伤口愈合试验评估细胞迁移/增殖；通过流式细胞术和RT-qPCR定量细胞骨架组织、细胞凋亡和坏死分析、活性氧产生、成骨标志物表达；通过茜素红S染色确定细胞矿化潜力。与在未处理培养基（阴性对照）和成骨培养基（阳性对照）中培养的hDPSCs进行比较。统计学显著性设定为p<0.05。
与阴性对照组相比，Biodentine XP（BD-XP）在细胞相容性检测中显示出显著的阳性结果，包括细胞代谢活性、活力、附着和形态，而MTA-Ang和Ther-PT显示出中等结果。与阴性和阳性对照组相比，BD-XP显示出成骨标志物（包括碱性磷酸酶和牙本质涎磷蛋白）的显著上调。在矿化潜力方面，与MTA-Ang、Ther-PT、阴性对照组和阳性对照组相比，BD-XP处理的细胞显示出显著更高的钙化结节形成。
BD-XP表现出优异的细胞相容性，并促进人牙髓干细胞（hDPSCs）的骨/牙源性分化。BD-XP显著增强了骨/牙源性标志物（如碱性磷酸酶和牙本质涎磷蛋白）的上调，并比MTA-Ang和Ther-PT更有效地促进钙化结节形成。BD-XP的高钙离子含量在其促进细胞黏附、增殖和矿化的能力中起关键作用，使其成为再生牙科应用中的高效材料。这些发现为支持在再生牙科治疗中使用Biodentine XP提供了有力证据，特别是在活髓治疗中，增强的细胞黏附和矿化对于临床成功至关重要。
