Ayoub Khaled Mohamed, Nagy Mohamed Mokhtar, Aly Riham Mohamed, El Deen Ghada Nour, El-Batouty Karim
Endodontic Department, Faculty of Dentistry, Badya University, Cairo, Egypt.
Endodontic Department, Faculty of Dentistry, Ain Shams University, Cairo, Egypt.
Sci Rep. 2025 Feb 8;15(1):4749. doi: 10.1038/s41598-025-88816-5.
The aim of the present study was to investigate the effects of the biological properties of hDPSCs exposed to Bio MTA+ & ProRoot MTA pulp capping materials on the proliferation and odontogenic differentiation of hDPSCs. Human dental pulp stem cells (hDPSCs) were isolated from impacted third molars. Extracts of Bio MTA + and ProRoot MTA were prepared at a 1:1 ratio. The effects of the extracts on hDPSCs cytotoxicity and proliferation were assessed via a CCK-8 assay. Annexin V expression was investigated to assess the effects of both materials on the induction of apoptosis. The effects of ProRoot MTA and Bio MTA + extraction media on the stemness properties of hDPSCs were assessed via real-time quantitative PCR, and the expression of odontogenic markers (RUNX2, DMP1 & DSSP) was analyzed via RT‒PCR Alizarin Red staining. Cells exposed to Bio MTA + had the greatest degree of proliferation. The results of Annexin V staining indicated that Bio MTA + caused the least amount of apoptosis. RUNX2, DMP1 and DSSP were highly expressed by Bio MTA + and indicated successful odontogenic differentiation. Compared with ProRoot MTA, Bio MTA + exhibited an exceptional level of cytocompatibility, as well as advantageous bioactivities, including the preservation of stemness and an increase in the proliferation capacity of hDPSCs. In addition, it demonstrated favorable bioactive properties by stimulating odontogenic differentiation. Bio MTA + offers significant advantages in terms of biocompatibility, bioactivity, and regenerative potential, making it an excellent choice for procedures aimed at preserving or regenerating dental pulp tissue. However, additional research is required to address the lack of in vivo validation, as replicating physiological conditions is crucial for accurately assessing clinical outcomes and comparing them with results obtained from in vitro experiments.
本研究的目的是探讨暴露于生物MTA+和ProRoot MTA盖髓材料的人牙髓干细胞（hDPSCs）的生物学特性对hDPSCs增殖和牙源性分化的影响。从阻生第三磨牙中分离出人牙髓干细胞（hDPSCs）。以1:1的比例制备生物MTA+和ProRoot MTA的提取物。通过CCK-8测定评估提取物对hDPSCs细胞毒性和增殖的影响。研究膜联蛋白V的表达以评估两种材料对细胞凋亡诱导的影响。通过实时定量PCR评估ProRoot MTA和生物MTA+提取培养基对hDPSCs干性特性的影响，并通过RT-PCR茜素红染色分析牙源性标志物（RUNX2、DMP1和DSSP）的表达。暴露于生物MTA+的细胞增殖程度最高。膜联蛋白V染色结果表明，生物MTA+引起的细胞凋亡最少。生物MTA+高表达RUNX2、DMP1和DSSP，表明牙源性分化成功。与ProRoot MTA相比，生物MTA+表现出卓越的细胞相容性以及有利的生物活性，包括保持干性和提高hDPSCs的增殖能力。此外，它通过刺激牙源性分化表现出良好的生物活性特性。生物MTA+在生物相容性、生物活性和再生潜力方面具有显著优势，使其成为旨在保存或再生牙髓组织的手术的极佳选择。然而，由于复制生理条件对于准确评估临床结果并将其与体外实验结果进行比较至关重要，因此需要进行更多研究来解决体内验证不足的问题。
