Li Yukuo, Song Zhe, Zhan Xu, Li Xiaohan, Ye Lingshuai, Lin Miaomiao, Wang Ran, Huang Hailei, Guo Jian, Sun Leiming, Gu Hong, Chen Jinyong, Fang Jinbao, Qi Xiujuan
National Key Laboratory for Germplasm Innovation & Utilization of Horticultural Crops, Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, 450009, China.
Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang, 453500, China.
Mol Hortic. 2025 Apr 1;5(1):18. doi: 10.1186/s43897-024-00139-7.
Actinidia arguta is a newly emerged, commercially cultivated Actinidia species. A. arguta has a beautiful appearance and is rich in anthocyanin, and is thus highly welcomed by consumers. However, the mechanism of anthocyanin regulation in A. arguta remains unclear. In this study, we assembled the nearly complete genome of the first red A. arguta cultivar, 'Tianyuanhong', with an N50 of 21 Mb. Comparative genome analysis revealed a role of the expansion/contraction of gene families in the species-specific trait formation of A. arguta. Through verification of transient overexpression and stable transformation, RNA-seq analysis revealed a key bHLH transcription factor, AaBEE1, which negatively regulates anthocyanin biosynthesis. DAP-seq analysis combined with Y1H, EMSA, Chip-qPCR and LUC suggested that AaBEE1 binds to the G-box of the AaLDOX promoter and suppresses its expression. Overall, we assembled the genome of A. arguta and clarified its AaBEE1-AaLDOX module-mediated molecular mechanism of anthocyanin regulation.
软枣猕猴桃是一种新出现的商业化栽培猕猴桃品种。软枣猕猴桃外观美观，富含花青素，因此深受消费者欢迎。然而，软枣猕猴桃中花青素调控的机制仍不清楚。在本研究中，我们组装了首个红色软枣猕猴桃品种‘天源红’的近完整基因组，N50为21 Mb。比较基因组分析揭示了基因家族的扩张/收缩在软枣猕猴桃物种特异性性状形成中的作用。通过瞬时过表达和稳定转化验证，RNA测序分析揭示了一个关键的bHLH转录因子AaBEE1，它对花青素生物合成起负调控作用。DNA亲和纯化测序分析结合酵母单杂交、电泳迁移率变动分析、染色质免疫沉淀定量PCR和荧光素酶实验表明，AaBEE1与AaLDOX启动子的G-box结合并抑制其表达。总体而言，我们组装了软枣猕猴桃的基因组，并阐明了其由AaBEE1-AaLDOX模块介导的花青素调控分子机制。
