Zhengzhou Fruit Research Institute, Chinese Academy of Agricultural Sciences, Zhengzhou, He Nan, 450009, China.
Sci Rep. 2017 Jul 18;7(1):5670. doi: 10.1038/s41598-017-06074-6.
Actinidia arguta 'Tianyuanhong' is a new kiwifruit variety with an all-red pericarp and pulp, in contrast to the all-green pulp of A. arguta 'Yongfengyihao'. Transcriptome profile analysis of fruit color has been reported, however, the metabolic mechanisms producing red flesh remain unknown, and it is unclear why the pulp of 'Tianyuanhong' is red rather than green. Herein, we identified differences between the proteomes of two A. arguta cultivars with different fruit color by using iTRAQ-based quantitative proteomic methods during the stage of color change. In total, 2310 differentially abundant proteins were detected between the two cultivars at 70 and 100 days after flowering, and the protein functions were analyzed based on KEGG and GO. The largest group of differentially expressed proteins were related to photosynthesis, glyoxylate metabolism, N metabolism, and anthocyanin biosynthesis. Finally, to verify the iTRAQ data, 12 representative genes encoding differentially expressed proteins were analyzed via quantitative real-time PCR, and these genes differed in transcriptional and translational expression levels. Our proteomic study contributes to understanding the metabolic pathways and biological processes involved in fruit color changes in different cultivars of A. arguta. These data and analyses will provide new insight into the development of kiwifruit flesh color.
软枣猕猴桃‘天源红’是一种果肉全红的新品种，与果肉全绿的‘永丰一号’形成鲜明对比。已有关于果实颜色转录组谱分析的报道，但产生红色果肉的代谢机制尚不清楚，也不清楚为什么‘天源红’的果肉是红色而不是绿色。在此，我们利用 iTRAQ 定量蛋白质组学方法，在花后 70 天和 100 天两个时期，对两个不同颜色果实的软枣猕猴桃品种进行了蛋白质组学差异分析。共检测到两个品种在花后 70 天和 100 天的差异表达蛋白 2310 个，基于 KEGG 和 GO 对这些蛋白的功能进行了分析。差异表达蛋白最大的一组与光合作用、乙醛酸代谢、N 代谢和花青素生物合成有关。最后，为了验证 iTRAQ 数据，通过定量实时 PCR 对 12 个编码差异表达蛋白的代表性基因进行了分析，这些基因在转录和翻译水平上的表达水平存在差异。本蛋白质组学研究有助于了解不同软枣猕猴桃品种果实颜色变化涉及的代谢途径和生物学过程。这些数据和分析将为猕猴桃果肉颜色的发展提供新的见解。
