Wang Xinyue, Li Jingwen, Zhou Yuxue, Zhang Jinhao, Wang Le, Liu Yajing, Yang Xuguang, Han Hongshuang, Wang Qingyu, Wang Ying
College of Plant Science, Jilin University, Changchun, China.
GM Crops Food. 2025 Dec;16(1):305-317. doi: 10.1080/21645698.2025.2486280. Epub 2025 Mar 31.
Biological nitrogen fixation (BNF) is the most cost-effective and environmentally benign method for nitrogen fertilization. Isoflavones are important signaling factors for BNF in leguminous plants. Whether chalcone isomerase (), the key enzyme gene in the flavonoid synthesis pathway, contributes to soybean () nodulation has not yet been fully clarified. In the present study, we identified the functions of three types of for BNF using a hairy root system. The results showed that and positively increased nodulation while did not, with the gene having a greater effect than . Meanwhile, the daidzein and genistein contents were significantly increased in composite plants overexpressing and reduced in composite plants, thus interfering with . However, overexpression of significantly increased the content of glycitein but not daidzein, genistein content implied that homologous genes exhibit functional differentiation. These results provide a reference for subsequent studies on improving nitrogen fixation in soybeans and providing functional genes for the improvement of new varieties.
生物固氮（BNF）是最具成本效益且环境友好的氮肥施用方法。异黄酮是豆科植物中生物固氮的重要信号因子。黄酮类合成途径中的关键酶基因查尔酮异构酶（）是否对大豆（）结瘤有贡献尚未完全阐明。在本研究中，我们利用发根系统鉴定了三种类型的 对生物固氮的功能。结果表明， 和 正向增加结瘤，而 则没有， 基因的作用比 更大。同时，过表达 和 的复合植株中大豆苷元和染料木黄酮含量显著增加，而在复合植株中含量降低，从而干扰了 。然而， 的过表达显著增加了黄豆黄素的含量，但未增加大豆苷元和染料木黄酮的含量，这表明同源基因表现出功能分化。这些结果为后续研究提高大豆固氮能力以及为新品种改良提供功能基因提供了参考。
